We have determined whether B cells previously activated by anti-Ig (anti-Ig blasts) are responsive to lymphokines that induce isotype switching. Culture of anti-Ig blasts with a mixture of lymphokines, including BSF-1, resulted in marked secretion of IgM and IgG1, but not other IgG isotypes. The IgG1 response of anti-Ig blasts to lymphokines was 13-fold greater than was observed with splenic B cells. B cell blasts induced by 8-mercaptoguanosine or dextran sulfate did not secrete high levels of any IgG isotype in response to lymphokines alone. An mAb against BSF-1 suppressed the IgG1 response of anti-Ig blasts, but not the IgM response to lymphokines. These data suggest that anti-Ig-treated B cells respond to at least one of the effects of BSF-1.
Article| July 01 1986
Antiimmunoglobulin-treated B cells respond to a B cell differentiation factor for IgG1.
P C Isakson
Online Issn: 1540-9538
Print Issn: 0022-1007
J Exp Med (1986) 164 (1): 303–308.
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P C Isakson; Antiimmunoglobulin-treated B cells respond to a B cell differentiation factor for IgG1.. J Exp Med 1 July 1986; 164 (1): 303–308. doi: https://doi.org/10.1084/jem.164.1.303
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