To determine why the H-2Dd antigen is expressed on the surface of transfected cells at a rate several-fold higher than an analogously transfected H-2Ld molecule, we analyzed both previously described and new H-2 hybrid genes. These genes were constructed by exchanging domains between H-2 genes. Quantitative radioimmunoassay indicates that the region of the H-2Dd molecule responsible for its enhanced expression resides in the polymorphic N domain, the first domain of the mature class I molecule.

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