Mouse chromosome 12 encodes the heavy chains of immunoglobulins (Igh), a family of T cell surface molecules, and a tumor antigen that may be homologous to immunoglobulins. To refine and extend the genetic map of this chromosome, a procedure has been developed to isolate chromosome 12-specific DNA fragments from a somatic cell hybrid carrying the chromosome on a Chinese hamster background. Five fragments have been isolated and characterized in detail. All are polymorphic, defining loci D12-1, 2, 3, 4, and 5. Using recombinant inbred mouse strains, a tentative linkage map of chromosome 12 has been worked out that incorporates these markers, the c-fos oncogene, Igh, and Pre-1/alpha 1 antitrypsin. This strategy should be applicable to any mouse chromosome or chromosomal region that can be isolated in a somatic cell hybrid.
Article| September 01 1984
A genetic map of mouse chromosome 12 composed of polymorphic DNA fragments.
Online Issn: 1540-9538
Print Issn: 0022-1007
J Exp Med (1984) 160 (3): 827–838.
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P D'Eustachio; A genetic map of mouse chromosome 12 composed of polymorphic DNA fragments.. J Exp Med 1 September 1984; 160 (3): 827–838. doi: https://doi.org/10.1084/jem.160.3.827
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