As opposed to normal human skin where HLA-DR expression is restricted to the Langerhans cell (LC) population, HLA-DR, but not HLA-DS antigens can be readily detected on keratinocytes (KC) in certain disease states, i.e., cutaneous T cell lymphoma (CTCL), graft-vs-host disease (GVHD), and lichen planus (LP). To clarify the cellular origin of KC-bound HLA-DR antigens, we used a monoclonal antibody directed against determinants solely expressed on the cytoplasmic HLA-DR gamma chain (VIC-Y1) and observed that, by immunofluorescence, KC displaying HLA-DR alpha/beta complexes on their surface uniformly displayed cytoplasmic VIC-Y1 reactivity. In view of the crucial role of the gamma chain for HLA-DR biosynthesis, we conclude that HLA-DR antigens on KC are actively synthesized by these cells.
Evidence of HLA-DR antigen biosynthesis by human keratinocytes in disease.
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B Volc-Platzer, O Majdic, W Knapp, K Wolff, W Hinterberger, K Lechner, G Stingl; Evidence of HLA-DR antigen biosynthesis by human keratinocytes in disease.. J Exp Med 1 June 1984; 159 (6): 1784–1789. doi: https://doi.org/10.1084/jem.159.6.1784
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