Human blood and tonsil B lymphocytes were fractionated on density gradients and tested for virus binding and penetration into the cells. Epstein-Barr Virus (EBV) transformation was detected by immunofluorescence staining for EBV-determined nuclear antigen (EBNA). EBV bound to and penetrated all B cell populations, but only the high density populations were transformed. Activated B lymphocytes were found in the low density fractions and these cells were resistant to EBV infection. Infected and noninfected B lymphocytes were density-analyzed during in vitro culture. A spontaneous, not virus-induced, density decrease was found to precede the production of EBNA. Cells remaining at high density never expressed EBNA. The results suggest that EBV can transform only small resting B lymphocytes and that a virus-independent activation of the infected cells induces the EBNA production and transformation.
Article| January 01 1984
Epstein-Barr virus susceptibility of normal human B lymphocyte populations.
Online Issn: 1540-9538
Print Issn: 0022-1007
J Exp Med (1984) 159 (1): 208–220.
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P Aman, B Ehlin-Henriksson, G Klein; Epstein-Barr virus susceptibility of normal human B lymphocyte populations.. J Exp Med 1 January 1984; 159 (1): 208–220. doi: https://doi.org/10.1084/jem.159.1.208
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