Nuclei of murine T lymphocytes or B lymphocytes were purified and transferred into lethally irradiated whole spleen cells or B or T lymphocytes by means of polyethyleneglycol-mediated cell fusion. Transfer of lymphocyte nuclei could save the irradiated cells from cell death, and such reconstituted cells could respond to mitogens. The present study showed that nuclei of T cells could be activated in the concanavalin A-stimulated T cell cytoplasms but not in the lipopolysaccharide-stimulated B cell cytoplasms. On the other hand, nuclei of B cells were activated in the lipopolysaccharide-stimulated B cells but not in the concanavalin A-stimulated T cell cytoplasms. These data suggested that a specific interaction between cytoplasm and nucleus might exist in the activation of nuclei of each lymphocyte subset.
Article| July 01 1982
Restricted nucleocytoplasmic relationship in activation of T and B lymphocytes.
Online Issn: 1540-9538
Print Issn: 0022-1007
J Exp Med (1982) 156 (1): 312–317.
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T Watanabe, Y Eda, J Ohara; Restricted nucleocytoplasmic relationship in activation of T and B lymphocytes.. J Exp Med 1 July 1982; 156 (1): 312–317. doi: https://doi.org/10.1084/jem.156.1.312
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