Spleen and other lymphoid tissues of rabbits immunized with human serum albumin (HSA) and human lactoferrin (LF) were examined for the presence of cells forming anti-idiotype antibodies. To detect these cells, IgG, F(ab')2, or Fab' of specific antibodies were isolated, fluorochrome-tagged with tetramethylrhodamine isothiocyanate, and used as an idiotypic marker to detect splenic plasma cells that are producing anti-idiotypic antibody. By this procedure, we were able to demonstrate anti-idiotypic cells in surprisingly high numbers. For example, in six rabbits immunized with HSA for periods ranging from 36 to 542 d, the percentage of Ig-positive cells that stained with autologous idiotype ranged from 0.7 to 44; furthermore, cross-reactivity was observed among seven different anti-HSA preparations and two anti-LF antisera. The isotype of anti-idiotypic cells, determined by costaining with fluorescein isothiocyanate-labeled goat Fc-specific anti-rabbit Ig, was shown to be predominantly IgG. These findings provide evidence of the presence of plasma cells producing antibody to autologous idiotype during a vigorous immune response.
Article| November 01 1979
Presence of plasma cells binding autologous antibody during an immune response.
Online Issn: 1540-9538
Print Issn: 0022-1007
J Exp Med (1979) 150 (5): 1265–1270.
- Views Icon Views
- PDF LinkPDF
- Share Icon Share
- Search Site
S Jackson, J Mestecky; Presence of plasma cells binding autologous antibody during an immune response.. J Exp Med 1 November 1979; 150 (5): 1265–1270. doi: https://doi.org/10.1084/jem.150.5.1265
Download citation file: