Human T-cell blasts were generated by stimulation with mitogens and antigens. A proportion of these blasts expressed Ia antigens detectable by immunofluorescence with both allo- and hetero-antiserums. The maximal expression of Ia antigens was delayed and usually occurred after the peak of blastogenesis. Among the three mitogens used, pokeweed mitogen (PWM) was most effective in giving a high percentage and intense Ia staining of T-cell blasts. Phytohemagglutinin and concanavalin A blasts gave weaker and lower percentages of Ia staining. Activation by alloantigens and soluble antigens such as tetanus toxoid and purified protein derivative resulted in Ia expression on T cells comparable to PWM stimulation. Depletion of Ia+ cells from freshly isolated T cells with anti-Ia and complement decreased subsequent Ia expression, suggesting that a proportion of Ia+ blasts were derived from Ia-bearing peripheral blood T cells. When the specificities of the Ia antigens on T-cell blasts were examined with alloantiserums, it was evident that the T blasts expressed similar HLA-DR determinants to those on B cells from the same donor; occasional minor differences between stimulated T cells and autologous B-cell lines or fresh B cells were encountered.
Ia determinants on stimulated human T lymphocytes. Occurrence on mitogen- and antigen-activated T cells.
- Views Icon Views
- PDF LinkPDF
- Share Icon Share
- Search Site
H S Ko, S M Fu, R J Winchester, D T Yu, H G Kunkel; Ia determinants on stimulated human T lymphocytes. Occurrence on mitogen- and antigen-activated T cells.. J Exp Med 1 August 1979; 150 (2): 246–255. doi: https://doi.org/10.1084/jem.150.2.246
Download citation file: