11 cultured human melanoma cell lines were tested for the expression of DR antigens by using specific allo- and xenoantisera in an indirect rosette microassay. Four of these melanoma cell lines expressed DR antigens, but in lower amounts than expressed on cultured human B-lymphoid cells. Rabbits injected with the DR-positive melanoma cells produced antibodies that were serologically and immunochemically reactive with B-cell-derived DR antigens. Immunochemical studies indicate that melanoma cell-derived DR antigens have a two-chain structure with 34,000 and 27,000 mol wt components. The melanoma cell-derived DR beta-chain at 27,000 mol wt is slightly smaller than that of the Victor cell DR beta-chain whose mol wt is 29,000.
DR (Ia-like) antigens on human melanoma cells. Serological detection and immunochemical characterization.
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B S Wilson, F Indiveri, M A Pellegrino, S Ferrone; DR (Ia-like) antigens on human melanoma cells. Serological detection and immunochemical characterization.. J Exp Med 1 March 1979; 149 (3): 658–668. doi: https://doi.org/10.1084/jem.149.3.658
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