Effector cells for cell-mediated lysis (CML) were generated by in vitro culture of lymphocytes from selected donors with X-irradiated cells from unrelated subjects who were HLA-D homozygous and matched to the responders for the antigens of the HLA-A and HLA-B regions. By using chromium labeled monocytes as target cells, cytotoxicity was found to correlate with presence of HLA-D region antigens matching those of the stimulating cells. Such CML reactions apparently directed at products of HLA-D, were inhibited by addition of unlabeled monocytes or B lymphocytes. These unlabeled cells had to be matched for HLA-D with the stimulating cells used to generate the effector populations. The results suggested that products of HLA-D, perhaps the DR antigens, were recognized by cytotoxic lymphocytes.
Article| February 01 1979
HLA-D region-associated determinants serve as targets for human cell-mediated lysis.
Online Issn: 1540-9538
Print Issn: 0022-1007
J Exp Med (1979) 149 (2): 485–494.
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C Feighery, P Stastny; HLA-D region-associated determinants serve as targets for human cell-mediated lysis.. J Exp Med 1 February 1979; 149 (2): 485–494. doi: https://doi.org/10.1084/jem.149.2.485
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