We have studied the modulation of Ia-like antigens on the surface membrane of human T cells responding in a one-way mixed leukocyte culture. A heterologous antiserum, (anti-p23,30), which is specific to HLA-D-related antigens and which is unreactive with normal peripheral T cells or thymocytes, was found to bind significantly to all T cells transformed in mixed leukocyte culture (MLC) as determined by indirect immunofluorescence on a fluorescence-activated cell sorter 1. Furthermore, cytotoxic T cells responsible for cell-mediated lympholysis were shown to react with anti-p23,30, whereas their unactivated progenitors did not. Immunoprecipitation and sodium dodecyl sulfate polyacrylamide gel electrophoresis of a radioactive 29,000 and 34,000 dalton complex from MLC-primed T cells labeled with [35S]methionine indicated that allosensitized T cells synthesized these HLA-D-related antigens.
Peripheral human T cells sensitized in mixed leukocyte culture synthesize and express Ia-like antigens.
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R L Evans, T J Faldetta, R E Humphreys, D M Pratt, E J Yunis, S F Schlossman; Peripheral human T cells sensitized in mixed leukocyte culture synthesize and express Ia-like antigens.. J Exp Med 1 November 1978; 148 (5): 1440–1445. doi: https://doi.org/10.1084/jem.148.5.1440
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