T cells from human peripheral blood was purified by fractionation on columns charged with human immunoglobulin and rabbit anti-human immuno-globulin. When assayed with 125I- or fluorescein isothiocyanate-labeled wheat-germ agglutinin (WGA), a weakly binding and a strongly binding subpopulation could be distinguished. These T-cell subpopulations were fractionated on columns charged with WGA, convalently bound to Sepharose 6MB. The cells responding to the mitogens leukoagglutinin from Phaseolus vulgaris and concanavalin A were enriched in the strongly binding subpopulation (approximately 20% of the T cells) while they were depleted from the weakly binding subpopulation.
Article| November 01 1976
Fractionation of human T lymphocytes on wheat germ agglutinin-sepharose.
M L Dillner
Online Issn: 1540-9538
Print Issn: 0022-1007
J Exp Med (1976) 144 (5): 1381–1385.
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U Hellström, M L Dillner, S Hammarström, P Perlmann; Fractionation of human T lymphocytes on wheat germ agglutinin-sepharose.. J Exp Med 2 November 1976; 144 (5): 1381–1385. doi: https://doi.org/10.1084/jem.144.5.1381
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