A quantitative assay that measures fibroblast chemotaxis in vitro is described. Application of this technique has revealed that peripheral blood lymphocytes stimulated by antigen or mitogen in vitro produce a factor that is chemotactic for human dermal fibroblasts. This lymphocyte-derived chemotactic factor for fibroblasts (LDCF-F) is different from the lymphokine that is chemotactic for monocytes or macrophages. Macrophages are required for the generation of LDCF-F by T lymphocytes stimulated by phytohemagglutinin. The fibroblast chemotactic factor is heat stable (56 degrees C for 30 min), trypsin sensitive, and neuraminidase resistant. LDCF-F could function to attact connective tissue fibroblasts to sites at which cell-mediated immune reactions are occurring in vivo.
Article|
November 01 1976
The chemotactic attraction of human fibroblasts to a lymphocyte-derived factor.
A E Postlethwaite
R Snyderman
A H Kang
Online Issn: 1540-9538
Print Issn: 0022-1007
J Exp Med (1976) 144 (5): 1188–1203.
Citation
A E Postlethwaite, R Snyderman, A H Kang; The chemotactic attraction of human fibroblasts to a lymphocyte-derived factor.. J Exp Med 2 November 1976; 144 (5): 1188–1203. doi: https://doi.org/10.1084/jem.144.5.1188
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