The relative susceptibility of neonatal and adult murine splenocytes to induction of B-cell tolerance was studied in vitro. Adult cells required approximately 1,000-fold more trinitrophenyl-human gamma globulin to be rendered tolerant than did cells from 9- to 12-day-old neonates. The potential effects of suppressor T cells were excluded by pretreating the cultured B cells with anti-Thy-1 and C' and the helper T cells with anti-Ly-2.2 and C'. The possible role of cell surface immunoglobulin isotypes in contributing to this observed difference is discussed.
Differential susceptibility of neonatal and adult murine spleen cells to in vitro induction of B-cell tolerance.
J C Cambier, J R Kettman, E S Vitetta, J W Uhr; Differential susceptibility of neonatal and adult murine spleen cells to in vitro induction of B-cell tolerance.. J Exp Med 1 July 1976; 144 (1): 293–297. doi: https://doi.org/10.1084/jem.144.1.293
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