Collagen, the major extracellular matrix protein, is also a membrane protein. Two types of collagen are detected on the normal human fibroblast membrane in culture, type I collagen and a new immunologically and chemically distinct collagen, type M (membrane) collagen. Antibodies to type M collagen elicited complement-mediated cytotoxicity, which could be blocked by pretreatment of the cells with bacterial collagenase or the antibody with type M collagen. Pretreatment of the cells with other proteolytic enzymes or the antibody with type I collagen or type III collagen had no effect on this complement-mediated cytotoxicity. Although type I collagen is the major collagen synthesized by normal human fibroblasts type M collagen may be the major cell membrane collagen and may be a major cell membrane component.
Immunologic characterization of the membrane-bound collagen in normal human fibroblasts: identification of a distinct membrane collagen.
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J R Lichtenstein, E A Bauer, R Hoyt, H J Wedner; Immunologic characterization of the membrane-bound collagen in normal human fibroblasts: identification of a distinct membrane collagen.. J Exp Med 1 July 1976; 144 (1): 145–154. doi: https://doi.org/10.1084/jem.144.1.145
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