A group of alloantibodies are found in pregnancy sera which react with antigens present on B lymphocytes and monocytes but are not detectable on the vast majority of unstimulated T cells. This specificity distinguishes them from HL-A antibodies which react with both cell types. They were readily recognized through indirect fluorescent antibody analysis by employing the combination of B-cell lymphoid lines and normal peripheral blood T cells. Different sera gave a variety of patterns of reactivity with a panel of 11 lymphoid lines. Similar differential patterns were also observed with normal B cells from different individuals particularly after concentrating the B cells. The antibodies were also cytotoxic to B cells and this procedure gave parallel results to the fluorescence method. The pattern of reactions obtained indicated a very heterogeneous system similar to that for HL-A. Special study of certain of the sera provided evidence that the lymphocyte-defined determinants of the mixed lymphocyte reaction system were involved. For convenience the term HL-B has been employed for these antigens.
Recognition by pregnancy serums of non-HL-A alloantigens selectively expressed on B lymphocytes.
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R J Winchester, P Wernet, H G Kunkel, B Dupont, C Jersild, S M Fu; Recognition by pregnancy serums of non-HL-A alloantigens selectively expressed on B lymphocytes.. J Exp Med 1 April 1975; 141 (4): 924–929. doi: https://doi.org/10.1084/jem.141.4.924
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