Passive transfer of thymocytes and spleen cells from donors primed with keyhole limpet hemocyanin (KLH) caused significant decrease in the average avidity of anti-DNP antibodies produced by direct and indirect PFC in the recipients in both primary and adoptive secondary antibody responses against DNP-KLH. The analysis of the avidity distribution of antibodies produced by plaque-forming cells (PFC) indicated that the observed decrease in the average avidity is primarily due to the selective loss of high avidity subpopulation of PFC leaving low avidity subpopulation relatively unaffected. The degree of suppression in antibody avidity did not correlate with the reduction in the number of PFC, and thus causing the "shift" of avidity distribution of PFC to the low avidity end. These results indicate that the "maturation" of antibody in the T-cell-dependent antibody response is influenced by the carrier-specific suppressor T cells with respect to the emergence and selection of B cells having high affinity receptors for hapten. It is suggested that B cells binding antigen with high affinity receptors would be more easily affected than those with low affinity receptors by specific suppressor T cells which are capable of reacting the carrier portion of the same antigen.
Article|
July 01 1974
SELECTIVE ROLES OF THYMUS-DERIVED LYMPHOCYTES IN THE ANTIBODY RESPONSE : II. PREFERENTIAL SUPPRESSION OF HIGH-AFFINITY ANTIBODY-FORMING CELLS BY CARRIER-PRIMED SUPPRESSOR T CELLS
Toshitada Takemori,
Toshitada Takemori
From the Department of Pathology, School of Medicine, Chiba University, Chiba, Japan
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Tomio Tada
Tomio Tada
From the Department of Pathology, School of Medicine, Chiba University, Chiba, Japan
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Toshitada Takemori
From the Department of Pathology, School of Medicine, Chiba University, Chiba, Japan
Tomio Tada
From the Department of Pathology, School of Medicine, Chiba University, Chiba, Japan
Received:
March 27 1974
Online Issn: 1540-9538
Print Issn: 0022-1007
Copyright © 1974 by The Rockefeller University Press
1974
J Exp Med (1974) 140 (1): 253–266.
Article history
Received:
March 27 1974
Citation
Toshitada Takemori, Tomio Tada; SELECTIVE ROLES OF THYMUS-DERIVED LYMPHOCYTES IN THE ANTIBODY RESPONSE : II. PREFERENTIAL SUPPRESSION OF HIGH-AFFINITY ANTIBODY-FORMING CELLS BY CARRIER-PRIMED SUPPRESSOR T CELLS . J Exp Med 1 July 1974; 140 (1): 253–266. doi: https://doi.org/10.1084/jem.140.1.253
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