The distribution, and quantity of immunoglobulins on the surface of lymphocytes has been studied by means of immunofluorescence and a quantitative radio-immunoassay. Surface immunoglobulins were found on approximately 45% of spleen and marrow lymphocytes and 7–14% of lymphocytes from lymph nodes, peripheral blood, and peritoneal exudate. Thymic lymphocytes contained undetectable amounts of immunoglobulin. In the spleen the different immunoglobulins were present in the following order: γG2 > γG1 > M > γA > γG3. The surface immunoglobulin was largely removable by brief treatment with trypsin. Quantitative analysis indicated that 50,000–150,000 molecules of immunoglobulin were present on an individual cell. A variety of observations make it likely that this lymphocyte-associated immunoglobulin. is a product of the cell to which it is attached rather than a form of cytophilic antibody.
IMMUNOGLOBULINS ON THE SURFACE OF LYMPHOCYTES : I. DISTRIBUTION AND QUANTITATION
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Enrique Rabellino, Sonia Colon, Howard M. Grey, Emil R. Unanue; IMMUNOGLOBULINS ON THE SURFACE OF LYMPHOCYTES : I. DISTRIBUTION AND QUANTITATION . J Exp Med 1 January 1971; 133 (1): 156–167. doi: https://doi.org/10.1084/jem.133.1.156
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