The restoration of immunological competence to irradiated adult mouse spleen was studied using an in vitro assay system. Competence of the spleen to evoke a graft versus host reaction in vitro was lost upon sublethal irradiation (550 R). Regeneration of immune competence occurred when such spleens were grown for 2–3 days in the presence of thymus tissue, but not when grown in the presence of a variety of other tissues. This thymic activity was demonstrated to occur across an intervening Millipore filter barrier. When lethal doses of irradiation were used, reactivation of immune competence did not occur unless both thymus and bone marrow were present. In this experimental situation the bone marrow appeared to supply the immunocompetent cells.
Article|
August 01 1967
REACTIVATION IN VITRO OF IMMUNOCOMPETENCE IN IRRADIATED MOUSE SPLEEN
Amiela Globerson,
Amiela Globerson
From the Department of Zoology, The University of Wisconsin, Madison, Wisconsin 53706
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Robert Auerbach
Robert Auerbach
From the Department of Zoology, The University of Wisconsin, Madison, Wisconsin 53706
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Amiela Globerson
From the Department of Zoology, The University of Wisconsin, Madison, Wisconsin 53706
Robert Auerbach
From the Department of Zoology, The University of Wisconsin, Madison, Wisconsin 53706
Received:
February 16 1967
Online Issn: 1540-9538
Print Issn: 0022-1007
Copyright © 1967 by The Rockefeller University Press
1967
J Exp Med (1967) 126 (2): 223–234.
Article history
Received:
February 16 1967
Citation
Amiela Globerson, Robert Auerbach; REACTIVATION IN VITRO OF IMMUNOCOMPETENCE IN IRRADIATED MOUSE SPLEEN . J Exp Med 1 August 1967; 126 (2): 223–234. doi: https://doi.org/10.1084/jem.126.2.223
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