Intravenous immunization of New Zealand red rabbits with streptococcal group-specific bacterial vaccines yielded sera which possessed markedly elevated γ-globulin. The sera of one rabbit immunized with Group A-variant vaccine possessed 55 mg/ml of γ-globulin. The bulk of this γ-globulin, identified as γG-globulin, was homogeneous by zone electrophoresis and of specificity directed against the Group A-variant carbohydrate antigen. L chains isolated from specific antibody obtained from an immune precipitate were distributed as a single band on starch gel electrophoresis, whereas the normal γ-globulin traveled as a diffuse smear. These data suggest that the rabbit streptococcal Group A-variant antibodies possess a limited range of physicochemical properties and electrophoretic mobility compared to that generally observed for the normal complement of γ-globulin.
CHARACTERISTICS OF STREPTOCOCCAL GROUP-SPECIFIC ANTIBODY ISOLATED FROM HYPERIMMUNE RABBITS
- Views Icon Views
- Share Icon Share
- Search Site
C. Kirk Osterland, Edward J. Miller, Walter W. Karakawa, Richard M. Krause; CHARACTERISTICS OF STREPTOCOCCAL GROUP-SPECIFIC ANTIBODY ISOLATED FROM HYPERIMMUNE RABBITS . J Exp Med 1 April 1966; 123 (4): 599–614. doi: https://doi.org/10.1084/jem.123.4.599
Download citation file: