STAINING TOXOPLASMA GONDII WITH FLUORESCEIN-LABELLED ANTIBODY I. THE REACTION IN SMEARS OF PERITONEAL EXUDATE

Goldman , Morris

doi:10.1084/jem.105.6.549

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Article| June 01 1957

STAINING TOXOPLASMA GONDII WITH FLUORESCEIN-LABELLED ANTIBODY : I. THE REACTION IN SMEARS OF PERITONEAL EXUDATE

Morris Goldman

From the United States Department of Health, Education, and Wdfare, Communicable Disease Center, Atlanta

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Author and Article Information

Morris Goldman

From the United States Department of Health, Education, and Wdfare, Communicable Disease Center, Atlanta

Received: February 03 1957

Online ISSN: 1540-9538

Print ISSN: 0022-1007

Copyright, 1957, by The Rockefeller Institute for Medical Research New York

1957

J Exp Med (1957) 105 (6): 549–556.

https://doi.org/10.1084/jem.105.6.549

Antitoxoplasma globulin was labelled with fluorescein and was used to stain Toxoplasma gondii organisms in smears of peritoneal exudate. The evidence indicates that this staining was due to an antigen-antibody reaction at the cellular level. Methods are presented for handling the organisms so that they can be stained over a period of several months. Some general characteristics of the antigen system involved are described.

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Copyright, 1957, by The Rockefeller Institute for Medical Research New York

1957

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STAINING TOXOPLASMA GONDII WITH FLUORESCEIN-LABELLED ANTIBODY : I. THE REACTION IN SMEARS OF PERITONEAL EXUDATE

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