Improvements in a method for the specific microscopic localization of antigen in tissue cells are described. This method employs antibody labelled with fluorescein isocyanate as a histochemical stain, the specific antigen-antibody precipitate being made visible under the fluorescence microscope.
Two isomeric series derived from nitrofluorescein are described.
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Copyright, 1950, by The Rockefeller Institute for Medical Research New York
1950
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