QUANTITATIVE CHEMICAL STUDIES ON COMPLEMENT OR ALEXIN III. UPTAKE OF COMPLEMENT NITROGEN UNDER VARYING EXPERIMENTAL CONDITIONS

Heidelberger , Michael; Rocha e Silva , M.; Mayer , Manfred

doi:10.1084/jem.74.4.359

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Article| October 01 1941

QUANTITATIVE CHEMICAL STUDIES ON COMPLEMENT OR ALEXIN : III. UPTAKE OF COMPLEMENT NITROGEN UNDER VARYING EXPERIMENTAL CONDITIONS

Michael Heidelberger,

Michael Heidelberger

From the Departments of Medicine and Biochemistry, College of Physicians and Surgeons, Columbia University, and the Presbyterian Hospital, New York

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M. Rocha e Silva,

M. Rocha e Silva

From the Departments of Medicine and Biochemistry, College of Physicians and Surgeons, Columbia University, and the Presbyterian Hospital, New York

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Manfred Mayer

From the Departments of Medicine and Biochemistry, College of Physicians and Surgeons, Columbia University, and the Presbyterian Hospital, New York

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Author and Article Information

Michael Heidelberger

From the Departments of Medicine and Biochemistry, College of Physicians and Surgeons, Columbia University, and the Presbyterian Hospital, New York

M. Rocha e Silva

From the Departments of Medicine and Biochemistry, College of Physicians and Surgeons, Columbia University, and the Presbyterian Hospital, New York

Manfred Mayer

From the Departments of Medicine and Biochemistry, College of Physicians and Surgeons, Columbia University, and the Presbyterian Hospital, New York

Received: June 26 1941

Online ISSN: 1540-9538

Print ISSN: 0022-1007

Copyright, 1941, by The Rockefeller Institute for Medical Research New York

1941

J Exp Med (1941) 74 (4): 359–367.

https://doi.org/10.1084/jem.74.4.359

1. Quantitative data are given on the effect of variations in the time of contact and the proportions of the reactants on the quantity of complement combining component nitrogen (C'1 N) found in active guinea pig serum.

2. C'1 N was the same when determined with precipitates containing excess antibody or excess antigen.

3. Finely divided specific precipitates took up the complement combining component (C'1) from subsequently added guinea pig serum almost as well as specific precipitates formed in the presence of complement.

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Copyright, 1941, by The Rockefeller Institute for Medical Research New York

1941

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QUANTITATIVE CHEMICAL STUDIES ON COMPLEMENT OR ALEXIN : III. UPTAKE OF COMPLEMENT NITROGEN UNDER VARYING EXPERIMENTAL CONDITIONS

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