By a method of differential centrifugation and tryptic digestion suspensions of elementary bodies have been prepared from chorioallantoic membranes of chick embryos infected with vaccine virus. The infective titer of the final suspension of elementary bodies was usually the same as that of the original tissue emulsion. Elementary bodies from infected chick membranes were agglutinated as well by antivaccinal serum obtained from different mammalian species as were bodies prepared from inoculated rabbit skin. Seitz filtrates of infected chick material contained soluble precipitable substances of vaccinia; these filtrates and filtrates from infected rabbit skin, respectively, reacted equally well with rabbit serum which contained either L or S antibodies.
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1 September 1937
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September 01 1937
ELEMENTARY BODIES OF VACCINIA FROM INFECTED CHORIO-ALLANTOIC MEMBRANES OF DEVELOPING CHICK EMBRYOS
Joseph E. Smadel,
Joseph E. Smadel
From the Hospital of The Rockefeller Institute for Medical Research
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M. J. Wall
M. J. Wall
From the Hospital of The Rockefeller Institute for Medical Research
Search for other works by this author on:
Joseph E. Smadel
From the Hospital of The Rockefeller Institute for Medical Research
M. J. Wall
From the Hospital of The Rockefeller Institute for Medical Research
Received:
June 14 1937
Online ISSN: 1540-9538
Print ISSN: 0022-1007
Copyright, 1937, by The Rockefeller Institute for Medical Research New York
1937
J Exp Med (1937) 66 (3): 325–336.
Article history
Received:
June 14 1937
Citation
Joseph E. Smadel, M. J. Wall; ELEMENTARY BODIES OF VACCINIA FROM INFECTED CHORIO-ALLANTOIC MEMBRANES OF DEVELOPING CHICK EMBRYOS . J Exp Med 1 September 1937; 66 (3): 325–336. doi: https://doi.org/10.1084/jem.66.3.325
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