The growth of a virulent Type I pneumococcus in an automatic transfer device on a medium which aerobically maintains virulence results in a decrease in the presence of pure oxygen or pure carbon dioxide, but in no change in the presence of nitrogen. Whereas partial pressures of oxygen, that is 5 parts with 95 parts nitrogen by volume, and 10 parts with 90 parts nitrogen, resulted in a decrease in virulence, with 20 parts oxygen and 80 parts nitrogen, the decrease was almost negligible. Media which under aerobic conditions failed to furnish suitable constituents for maintenance of virulence, that is spleen, aminoid peptone, powdered milk, beef infusion, whole rabbit, and rabbit muscle, also failed under nitrogen. Media from whole guinea pig kept the organism virulent for 246 transfers under nitrogen, but with some decrease. Media made from discard mice, survivors of pneumococcus infection, furnished conditions under nitrogen, which caused first a decrease of virulence, but later after 246 transfers, a restoration to a more virulent state. Gradual increase in temperature over a period of 10 days from 36.5°C. to 42°C. in a sample of medium, which was otherwise suitable for maintenance of virulence under aerobic conditions, resulted in a decrease in virulence.
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1 July 1932
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July 01 1932
STUDIES ON VIRULENCE : V. THE EFFECT OF OXYGEN, NITROGEN, AND CARBON DIOXIDE ON THE VIRULENCE OF GROWING PNEUMOCOCCI
Lloyd D. Felton
Lloyd D. Felton
From the Department of Preventive Medicine and Hygiene, Harvard Medical School, Boston
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Lloyd D. Felton
From the Department of Preventive Medicine and Hygiene, Harvard Medical School, Boston
Received:
April 03 1932
Online ISSN: 1540-9538
Print ISSN: 0022-1007
Copyright, 1932, by The Rockefeller Institute for Medical Research New York
1932
J Exp Med (1932) 56 (1): 27–38.
Article history
Received:
April 03 1932
Citation
Lloyd D. Felton; STUDIES ON VIRULENCE : V. THE EFFECT OF OXYGEN, NITROGEN, AND CARBON DIOXIDE ON THE VIRULENCE OF GROWING PNEUMOCOCCI . J Exp Med 1 July 1932; 56 (1): 27–38. doi: https://doi.org/10.1084/jem.56.1.27
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