THE REMOVAL OF AGGLUTININ FROM SENSITIZED MOTILE BACTERIA

It was shown that flocculating (flagellar) agglutinin and granulating (somatic) agglutinin display certain differences with respect to their removal from sensitized bacteria (B. paratyphi). A 5 per cent solution of NaCl added to sedimented, sensitized bacteria followed by heating to 60°C. for 1 hour removed approximately 50 per cent of the combined agglutinin. There was little or no removal of granulating agglutinin either from the sensitized motile bacteria or from a sensitized non-motile organism (Staphylococcus). Evidence was presented that the agglutinin removal was not dependent solely on disintegration of flagella by the conditions of extraction with a subsequent freeing of antibody.