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Volume 42, Issue 6
1 December 1925
Journal of Experimental Medicine Cover Image for Volume 42, Issue 6
Article| December 01 1925

THE IMMUNIZING ANTIGEN OF THE PNEUMOCOCCUS : III. THE PURIFICATION OF THE WATER-SOLUBLE ANTIGEN.

William A. Perlzweig,
William A. Perlzweig
From the Chemical Division of the Medical Clinic of the Johns Hopkins University and Hospital, Baltimore.
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Chester S. Keefer
Chester S. Keefer
From the Chemical Division of the Medical Clinic of the Johns Hopkins University and Hospital, Baltimore.
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Author and Article Information
William A. Perlzweig
From the Chemical Division of the Medical Clinic of the Johns Hopkins University and Hospital, Baltimore.
Chester S. Keefer
From the Chemical Division of the Medical Clinic of the Johns Hopkins University and Hospital, Baltimore.
Received: August 12 1925
Online ISSN: 1540-9538
Print ISSN: 0022-1007
Copyright, 1925, by The Rockefeller Institute for Medical Research New York
1925
J Exp Med (1925) 42 (6): 747–752.
https://doi.org/10.1084/jem.42.6.747
Article history
Received:
August 12 1925

Actively immunizing fractions of protein nature have been isolated from broth culture filtrates of Pneumococcus Type I by ultrafiltration, precipitation at a definite hydrion concentration, and the separation of a soluble picrate fraction. The method appears to be suitable for the initial purification of this antigen.

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Copyright, 1925, by The Rockefeller Institute for Medical Research New York
1925
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