Langerhans cells (LC) and other antigen-presenting cells are believed to be critical in initiating graft versus host responses that influence the outcome of allogeneic hematopoietic stem cell transplantation. However, their fate in humans is poorly understood. We have sought to define the effect of conditioning regimes and graft versus host disease (GVHD) on the survival of recipient LC and reconstitution of donor cells after transplant. Confocal microscopy of epidermal sheets shows that full intensity transplant (FIT) depletes LC more rapidly than reduced intensity transplant (RIT) at day 0, although the nadir is similar in both at 14–21 d. Recovery occurs rapidly within 40 d in the absence of acute GVHD, but is delayed beyond 100 d when GVHD is active. LC chimerism was determined in sex-mismatched transplants using a two-step Giemsa/fluorescence in situ hybridization assay on isolated cells. Acquisition of donor chimerism at 40 d is more rapid after FIT (97%) than RIT (36.5%), irrespective of blood myeloid engraftment. At 100 d, all transplants achieve at least 90% LC donor chimerism and over half achieve 100%. Complete donor chimerism is associated with prior acute cutaneous GVHD, suggesting a role for allogeneic T cells in promoting LC engraftment.
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23 January 2006
Brief Definitive Report|
January 03 2006
The fate of human Langerhans cells in hematopoietic stem cell transplantation
Matthew P. Collin,
Matthew P. Collin
1Haematological Sciences, University of Newcastle, Newcastle upon Tyne NE2 4HH, England, UK
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Derek N.J. Hart,
Derek N.J. Hart
2Mater Medical Research Institute, South Brisbane, Queensland 4101, Australia
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Graham H. Jackson,
Graham H. Jackson
1Haematological Sciences, University of Newcastle, Newcastle upon Tyne NE2 4HH, England, UK
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Gordon Cook,
Gordon Cook
3Department of Haematology, St. James's University Hospital, Leeds LS9 7TF, England, UK
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James Cavet,
James Cavet
4Department of Haematology, Christie Hospital NHS Trust, Manchester M20 4BX, England, UK
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Stephen Mackinnon,
Stephen Mackinnon
5Department of Haematology, Royal Free and University College London Medical School, London NW3 2QG, England, UK
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Peter G. Middleton,
Peter G. Middleton
1Haematological Sciences, University of Newcastle, Newcastle upon Tyne NE2 4HH, England, UK
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Anne M. Dickinson
Anne M. Dickinson
1Haematological Sciences, University of Newcastle, Newcastle upon Tyne NE2 4HH, England, UK
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Matthew P. Collin
1Haematological Sciences, University of Newcastle, Newcastle upon Tyne NE2 4HH, England, UK
Derek N.J. Hart
2Mater Medical Research Institute, South Brisbane, Queensland 4101, Australia
Graham H. Jackson
1Haematological Sciences, University of Newcastle, Newcastle upon Tyne NE2 4HH, England, UK
Gordon Cook
3Department of Haematology, St. James's University Hospital, Leeds LS9 7TF, England, UK
James Cavet
4Department of Haematology, Christie Hospital NHS Trust, Manchester M20 4BX, England, UK
Stephen Mackinnon
5Department of Haematology, Royal Free and University College London Medical School, London NW3 2QG, England, UK
Peter G. Middleton
1Haematological Sciences, University of Newcastle, Newcastle upon Tyne NE2 4HH, England, UK
Anne M. Dickinson
1Haematological Sciences, University of Newcastle, Newcastle upon Tyne NE2 4HH, England, UK
CORRESPONDENCE Matthew Collin: [email protected]
Received:
September 02 2005
Accepted:
December 01 2005
Online ISSN: 1540-9538
Print ISSN: 0022-1007
The Rockefeller University Press
2006
J Exp Med (2006) 203 (1): 27–33.
Article history
Received:
September 02 2005
Accepted:
December 01 2005
Citation
Matthew P. Collin, Derek N.J. Hart, Graham H. Jackson, Gordon Cook, James Cavet, Stephen Mackinnon, Peter G. Middleton, Anne M. Dickinson; The fate of human Langerhans cells in hematopoietic stem cell transplantation . J Exp Med 23 January 2006; 203 (1): 27–33. doi: https://doi.org/10.1084/jem.20051787
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