The development of an immune response critically relies on the encounter of rare antigen (Ag)-specific T cells with dendritic cells (DCs) presenting the relevant Ag. How two rare cells find each other in the midst of irrelevant other cells in lymph nodes (LNs) is unknown. Here we show that initial T cell activation clusters are generated near high endothelial venules (HEVs) in the outer paracortex of draining LNs by retention of Ag-specific T cells as they exit from HEVs. We further show that tissue-derived DCs preferentially home in the vicinity of HEVs, thus defining the site of cluster generation. At this location DCs efficiently scan all incoming T cells and selectively retain those specific for the major histocompatibility complex–peptide complexes the DCs present. Such strategic positioning of DCs on the entry route of T cells into the paracortex may foster T cell–DC encounter and thus optimize initial T cell activation in vivo.
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1 September 2003
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September 02 2003
The Strategy of T Cell Antigen-presenting Cell Encounter in Antigen-draining Lymph Nodes Revealed by Imaging of Initial T Cell Activation
Marc Bajénoff,
Marc Bajénoff
1Centre d'Immunologie de Marseille-Luminy, Institut National de la Santé et de la Recherche Médicale (INSERM)/Centre National de la Recherche Scientifique (CNRS), Université de la Méditerranée, 13288 Marseille, France
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Samuel Granjeaud,
Samuel Granjeaud
2IFR57, TAGC, INSERM-ERM206, 13288 Marseille, France
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Sylvie Guerder
Sylvie Guerder
1Centre d'Immunologie de Marseille-Luminy, Institut National de la Santé et de la Recherche Médicale (INSERM)/Centre National de la Recherche Scientifique (CNRS), Université de la Méditerranée, 13288 Marseille, France
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Marc Bajénoff
1Centre d'Immunologie de Marseille-Luminy, Institut National de la Santé et de la Recherche Médicale (INSERM)/Centre National de la Recherche Scientifique (CNRS), Université de la Méditerranée, 13288 Marseille, France
Samuel Granjeaud
2IFR57, TAGC, INSERM-ERM206, 13288 Marseille, France
Sylvie Guerder
1Centre d'Immunologie de Marseille-Luminy, Institut National de la Santé et de la Recherche Médicale (INSERM)/Centre National de la Recherche Scientifique (CNRS), Université de la Méditerranée, 13288 Marseille, France
Address correspondence to Sylvie Guerder, Centre d'Immunologie de Marseille-Luminy, INSERM/Centre National de la Recherche Scientifique, Université de la Méditerranée, Parc Scientifique de Luminy, Case 906, 13288 Marseille Cedex 09, France. Phone: 33-4-91-26-94-07; Fax: 33-4-91-26-94-30; email: [email protected]
Abbreviations used in this paper: CFSE, 5-(and-6)-carboxyfluorescein diacetate succinimidyl ester; CMTMR, orange fluorescent tetramethylrhodamine; HEL, hen egg lysozyme; HEV, high endothelial venule; PCC, pigeon cytochrome c; PNAd, peripheral LN addressin.
The online version of this article contains supplemental material.
Received:
January 31 2003
Revision Received:
June 30 2003
Accepted:
June 30 2003
Online ISSN: 1540-9538
Print ISSN: 0022-1007
The Rockefeller University Press
2003
J Exp Med (2003) 198 (5): 715–724.
Article history
Received:
January 31 2003
Revision Received:
June 30 2003
Accepted:
June 30 2003
Citation
Marc Bajénoff, Samuel Granjeaud, Sylvie Guerder; The Strategy of T Cell Antigen-presenting Cell Encounter in Antigen-draining Lymph Nodes Revealed by Imaging of Initial T Cell Activation . J Exp Med 1 September 2003; 198 (5): 715–724. doi: https://doi.org/10.1084/jem.20030167
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