Limited frequencies of T cells express IL-2 in primary antigenic responses, despite activation marker expression and proliferation by most clonal members. To define the basis for restricted IL-2 expression, a videomicroscopic system and IL-2 reporter transgenic model were used to characterize dendritic cell (DC)–T cell interactions. T cells destined to produce IL-2 required prolonged interactions with DCs, whereas most T cells established only transient interactions with DCs and were activated, but did not express IL-2. Extended conjugation of T cells with DCs was not always sufficient to initiate IL-2 expression. Thus, there is intrinsic variability in clonal T cell populations that restricts IL-2 commitment, and prolonged engagement with mature DCs is necessary, but not sufficient, for IL-2 gene transcription.
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7 July 2003
Article|
June 30 2003
Restricted Clonal Expression of IL-2 By Naive T Cells Reflects Differential Dynamic Interactions with Dendritic Cells
Vincent Hurez,
Vincent Hurez
1Department of Pathology, University of Alabama at Birmingham, Birmingham, AL 35294
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Arman Saparov,
Arman Saparov
1Department of Pathology, University of Alabama at Birmingham, Birmingham, AL 35294
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Albert Tousson,
Albert Tousson
2Department of Cell Biology, University of Alabama at Birmingham, Birmingham, AL 35294
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Michael J. Fuller,
Michael J. Fuller
2Department of Cell Biology, University of Alabama at Birmingham, Birmingham, AL 35294
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Takekazu Kubo,
Takekazu Kubo
1Department of Pathology, University of Alabama at Birmingham, Birmingham, AL 35294
4Biological Research Laboratories, Sankyo Co., Ltd., Tokyo 140-8710, Japan
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James Oliver,
James Oliver
1Department of Pathology, University of Alabama at Birmingham, Birmingham, AL 35294
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Benjamin T. Weaver,
Benjamin T. Weaver
1Department of Pathology, University of Alabama at Birmingham, Birmingham, AL 35294
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Casey T. Weaver
Casey T. Weaver
1Department of Pathology, University of Alabama at Birmingham, Birmingham, AL 35294
3Department of Microbiology, University of Alabama at Birmingham, Birmingham, AL 35294
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Vincent Hurez
1Department of Pathology, University of Alabama at Birmingham, Birmingham, AL 35294
Arman Saparov
1Department of Pathology, University of Alabama at Birmingham, Birmingham, AL 35294
Albert Tousson
2Department of Cell Biology, University of Alabama at Birmingham, Birmingham, AL 35294
Michael J. Fuller
2Department of Cell Biology, University of Alabama at Birmingham, Birmingham, AL 35294
Takekazu Kubo
1Department of Pathology, University of Alabama at Birmingham, Birmingham, AL 35294
4Biological Research Laboratories, Sankyo Co., Ltd., Tokyo 140-8710, Japan
James Oliver
1Department of Pathology, University of Alabama at Birmingham, Birmingham, AL 35294
Benjamin T. Weaver
1Department of Pathology, University of Alabama at Birmingham, Birmingham, AL 35294
Casey T. Weaver
1Department of Pathology, University of Alabama at Birmingham, Birmingham, AL 35294
3Department of Microbiology, University of Alabama at Birmingham, Birmingham, AL 35294
Address correspondence to Casey T. Weaver, 870 Bevill Biomedical Research Building, 845 19th St. South, Birmingham, AL 35294-2170. Phone: 205-975-5537; Fax: 205-975-8310; E-mail: [email protected]
The online version of this article includes supplemental material.
*
Abbreviations used in this paper: BM, bone marrow; DC, dendritic cell; GFP, green fluorescent protein; LTC, long-term conjugate.
Received:
December 30 2002
Revision Received:
May 08 2003
Accepted:
May 08 2003
Online ISSN: 1540-9538
Print ISSN: 0022-1007
The Rockefeller University Press
2003
J Exp Med (2003) 198 (1): 123–132.
Article history
Received:
December 30 2002
Revision Received:
May 08 2003
Accepted:
May 08 2003
Citation
Vincent Hurez, Arman Saparov, Albert Tousson, Michael J. Fuller, Takekazu Kubo, James Oliver, Benjamin T. Weaver, Casey T. Weaver; Restricted Clonal Expression of IL-2 By Naive T Cells Reflects Differential Dynamic Interactions with Dendritic Cells . J Exp Med 7 July 2003; 198 (1): 123–132. doi: https://doi.org/10.1084/jem.20022230
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