Immunoglobulin light chain (IgL) rearrangements occur more frequently at Igκ than at Igλ. Previous results suggested that the unrearranged Igκ locus negatively regulates Igλ transcription and/or rearrangement. Here, we demonstrate that expression of a VJλ1-joint inserted into its physiological position in the Igλ locus is independent of Igκ rearrangements. Expression of the inserted VJλ1 gene segment is developmentally controlled like that of a VJκ-joint inserted into the Igκ locus and furthermore coincides developmentally with the occurrence of Igκ rearrangements in wild-type mice. We conclude that developmentally controlled transcription of a gene rearrangement in the Igλ locus occurs in the presence of an unrearranged Igκ locus and is therefore not negatively regulated by the latter. Our data also indicate light chain editing in ∼30% of λ1 expressing B cell progenitors.
Skip Nav Destination
Article navigation
5 May 2003
Article|
April 28 2003
Expression of a Targeted λ1 Light Chain Gene Is Developmentally Regulated and Independent of Igκ Rearrangements
Philipp Oberdoerffer,
Philipp Oberdoerffer
1Center for Blood Research, Harvard Medical School, 200 Longwood Avenue, Boston, MA 02115
2Institute for Genetics, University of Cologne, 121 Weyertal, 50932 Cologne, Germany
Search for other works by this author on:
Tatiana I. Novobrantseva,
Tatiana I. Novobrantseva
1Center for Blood Research, Harvard Medical School, 200 Longwood Avenue, Boston, MA 02115
2Institute for Genetics, University of Cologne, 121 Weyertal, 50932 Cologne, Germany
Search for other works by this author on:
Klaus Rajewsky
Klaus Rajewsky
1Center for Blood Research, Harvard Medical School, 200 Longwood Avenue, Boston, MA 02115
2Institute for Genetics, University of Cologne, 121 Weyertal, 50932 Cologne, Germany
Search for other works by this author on:
Philipp Oberdoerffer
1Center for Blood Research, Harvard Medical School, 200 Longwood Avenue, Boston, MA 02115
2Institute for Genetics, University of Cologne, 121 Weyertal, 50932 Cologne, Germany
Tatiana I. Novobrantseva
1Center for Blood Research, Harvard Medical School, 200 Longwood Avenue, Boston, MA 02115
2Institute for Genetics, University of Cologne, 121 Weyertal, 50932 Cologne, Germany
Klaus Rajewsky
1Center for Blood Research, Harvard Medical School, 200 Longwood Avenue, Boston, MA 02115
2Institute for Genetics, University of Cologne, 121 Weyertal, 50932 Cologne, Germany
Address correspondence to K. Rajewsky, Center for Blood Research, Harvard Medical School, 200 Longwood Ave., Boston, MA 02115. Phone: 617-278-3067; Fax: 617-278-3129; E-mail: [email protected]
T.I. Novobrantseva's present address is Biogen Inc., 14 Cambridge Center, Cambridge, MA 02142.
*
Abbreviations used in this paper: ES, embryonic stem; RSS, recombination signal sequence.
Received:
March 13 2003
Revision Received:
March 13 2003
Accepted:
March 23 2003
Online ISSN: 1540-9538
Print ISSN: 0022-1007
The Rockefeller University Press
2003
J Exp Med (2003) 197 (9): 1165–1172.
Article history
Received:
March 13 2003
Revision Received:
March 13 2003
Accepted:
March 23 2003
Connected Content
This article has been corrected
Correction
Citation
Philipp Oberdoerffer, Tatiana I. Novobrantseva, Klaus Rajewsky; Expression of a Targeted λ1 Light Chain Gene Is Developmentally Regulated and Independent of Igκ Rearrangements . J Exp Med 5 May 2003; 197 (9): 1165–1172. doi: https://doi.org/10.1084/jem.20030402
Download citation file:
Sign in
Don't already have an account? Register
Client Account
You could not be signed in. Please check your email address / username and password and try again.
Could not validate captcha. Please try again.
Sign in via your Institution
Sign in via your InstitutionEmail alerts
Connected Content
Advertisement