Dengue virus is a single-stranded, enveloped RNA virus that productively infects human dendritic cells (DCs) primarily at the immature stage of their differentiation. We now find that all four serotypes of dengue use DC-SIGN (CD209), a C-type lectin, to infect dendritic cells. THP-1 cells become susceptible to dengue infection after transfection of DC-specific ICAM-3 grabbing nonintegrin (DC-SIGN), or its homologue L-SIGN, whereas the infection of dendritic cells is blocked by anti–DC-SIGN antibodies and not by antibodies to other molecules on these cells. Viruses produced by dendritic cells are infectious for DC-SIGN– and L-SIGN–bearing THP-1 cells and other permissive cell lines. Therefore, DC-SIGN may be considered as a new target for designing therapies that block dengue infection.
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7 April 2003
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April 07 2003
DC-SIGN (CD209) Mediates Dengue Virus Infection of Human Dendritic Cells
Boonrat Tassaneetrithep,
Boonrat Tassaneetrithep
1Division of Retrovirology, Walter Reed Army Institute of Research and Henry M. Jackson Foundation for the Advancement of Military Medicine Diseases, Rockville, MD 20850
6Department of Immunology, Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand
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Timothy H. Burgess,
Timothy H. Burgess
2Viral Diseases Department, Naval Medical Research Center, Silver Spring, MD 20889
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Angela Granelli-Piperno,
Angela Granelli-Piperno
3Laboratory of Cellular Physiology and Immunology, The Rockefeller University, New York, NY 10021
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Christine Trumpfheller,
Christine Trumpfheller
3Laboratory of Cellular Physiology and Immunology, The Rockefeller University, New York, NY 10021
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Jennifer Finke,
Jennifer Finke
3Laboratory of Cellular Physiology and Immunology, The Rockefeller University, New York, NY 10021
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Wellington Sun,
Wellington Sun
4Department of Virus Diseases, Walter Reed Army Institute of Research, Silver Spring, MD 20889
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Michael A. Eller,
Michael A. Eller
1Division of Retrovirology, Walter Reed Army Institute of Research and Henry M. Jackson Foundation for the Advancement of Military Medicine Diseases, Rockville, MD 20850
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Kovit Pattanapanyasat,
Kovit Pattanapanyasat
5Division of Instruments for Research, Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand
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Suttipant Sarasombath,
Suttipant Sarasombath
6Department of Immunology, Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand
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Deborah L. Birx,
Deborah L. Birx
1Division of Retrovirology, Walter Reed Army Institute of Research and Henry M. Jackson Foundation for the Advancement of Military Medicine Diseases, Rockville, MD 20850
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Ralph M. Steinman,
Ralph M. Steinman
3Laboratory of Cellular Physiology and Immunology, The Rockefeller University, New York, NY 10021
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Sarah Schlesinger,
Sarah Schlesinger
3Laboratory of Cellular Physiology and Immunology, The Rockefeller University, New York, NY 10021
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Mary A. Marovich
Mary A. Marovich
1Division of Retrovirology, Walter Reed Army Institute of Research and Henry M. Jackson Foundation for the Advancement of Military Medicine Diseases, Rockville, MD 20850
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Boonrat Tassaneetrithep
1Division of Retrovirology, Walter Reed Army Institute of Research and Henry M. Jackson Foundation for the Advancement of Military Medicine Diseases, Rockville, MD 20850
6Department of Immunology, Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand
Timothy H. Burgess
2Viral Diseases Department, Naval Medical Research Center, Silver Spring, MD 20889
Angela Granelli-Piperno
3Laboratory of Cellular Physiology and Immunology, The Rockefeller University, New York, NY 10021
Christine Trumpfheller
3Laboratory of Cellular Physiology and Immunology, The Rockefeller University, New York, NY 10021
Jennifer Finke
3Laboratory of Cellular Physiology and Immunology, The Rockefeller University, New York, NY 10021
Wellington Sun
4Department of Virus Diseases, Walter Reed Army Institute of Research, Silver Spring, MD 20889
Michael A. Eller
1Division of Retrovirology, Walter Reed Army Institute of Research and Henry M. Jackson Foundation for the Advancement of Military Medicine Diseases, Rockville, MD 20850
Kovit Pattanapanyasat
5Division of Instruments for Research, Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand
Suttipant Sarasombath
6Department of Immunology, Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand
Deborah L. Birx
1Division of Retrovirology, Walter Reed Army Institute of Research and Henry M. Jackson Foundation for the Advancement of Military Medicine Diseases, Rockville, MD 20850
Ralph M. Steinman
3Laboratory of Cellular Physiology and Immunology, The Rockefeller University, New York, NY 10021
Sarah Schlesinger
3Laboratory of Cellular Physiology and Immunology, The Rockefeller University, New York, NY 10021
Mary A. Marovich
1Division of Retrovirology, Walter Reed Army Institute of Research and Henry M. Jackson Foundation for the Advancement of Military Medicine Diseases, Rockville, MD 20850
Address correspondence to Mary A. Marovich, 13 Taft Ct., Suite 200, Rockville, MD 20850. Phone: 301 251-8337; Fax: 301 762-4177; E-mail: [email protected]
R.M. Steinman, S. Schlesinger, and M.A. Marovich all contributed equally to this work.
*
Abbreviations used in this paper: DC, dendritic cell; DC-SIGN, DC-specific ICAM-3 grabbing nonintegrin; DV, dengue virus; HIV-1, HIV-type 1; ICAM, intracellular adhesion molecule; L-SIGN, liver/lymph node–specific ICAM-3–grabbing nonintegrin; MCM, monocyte-conditioned media; MFI, mean fluorescence intensity; MOI, multiplicity of infection; NS1, nonstructural protein 1; THP DC-SIGN, DC-SIGN–transfected THP.
Received:
October 22 2002
Revision Received:
December 29 2002
Accepted:
January 28 2003
Online ISSN: 1540-9538
Print ISSN: 0022-1007
The Rockefeller University Press
2003
J Exp Med (2003) 197 (7): 823–829.
Article history
Received:
October 22 2002
Revision Received:
December 29 2002
Accepted:
January 28 2003
Citation
Boonrat Tassaneetrithep, Timothy H. Burgess, Angela Granelli-Piperno, Christine Trumpfheller, Jennifer Finke, Wellington Sun, Michael A. Eller, Kovit Pattanapanyasat, Suttipant Sarasombath, Deborah L. Birx, Ralph M. Steinman, Sarah Schlesinger, Mary A. Marovich; DC-SIGN (CD209) Mediates Dengue Virus Infection of Human Dendritic Cells . J Exp Med 7 April 2003; 197 (7): 823–829. doi: https://doi.org/10.1084/jem.20021840
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