In this study we have shown that activation of arthritogenic splenocytes with antigen and agonistic anti-CD40 gives raise to a B cell population that produce high levels of interleukin (IL)-10 and low levels of interferon (IFN)-γ. Transfer of these B cells into DBA/1-TcR-β-Tg mice, immunized with bovine collagen (CII) emulsified in complete Freund's adjuvant inhibited T helper type 1 differentiation, prevented arthritis development, and was also effective in ameliorating established disease. IL-10 is essential for the regulatory function of this subset of B cells, as the B cells population isolated from IL-10 knockout mice failed to mediate this protective function. Furthermore, B cells isolated from arthritogenic splenocytes treated in vitro with anti–IL-10/anti–IL-10R were unable to protect recipient mice from developing arthritis. Our results suggest a new role of a subset of B cells in controlling T cell differentiation and autoimmune disorders.
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17 February 2003
Article|
February 10 2003
Prevention of Arthritis by Interleukin 10–producing B Cells
Claudia Mauri,
Claudia Mauri
1The Kennedy Institute of Rheumatology Division, Faculty of Medicine, Imperial College of Science Technology and Medicine, London W6 8 LH, United Kingdom
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David Gray,
David Gray
2Institute of Cell Animal and Population Biology, University of Edinburgh, Ashworth Laboratories, Edinburgh EH93JT, United Kingdom
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Naseem Mushtaq,
Naseem Mushtaq
1The Kennedy Institute of Rheumatology Division, Faculty of Medicine, Imperial College of Science Technology and Medicine, London W6 8 LH, United Kingdom
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Marco Londei
Marco Londei
1The Kennedy Institute of Rheumatology Division, Faculty of Medicine, Imperial College of Science Technology and Medicine, London W6 8 LH, United Kingdom
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Claudia Mauri
1The Kennedy Institute of Rheumatology Division, Faculty of Medicine, Imperial College of Science Technology and Medicine, London W6 8 LH, United Kingdom
David Gray
2Institute of Cell Animal and Population Biology, University of Edinburgh, Ashworth Laboratories, Edinburgh EH93JT, United Kingdom
Naseem Mushtaq
1The Kennedy Institute of Rheumatology Division, Faculty of Medicine, Imperial College of Science Technology and Medicine, London W6 8 LH, United Kingdom
Marco Londei
1The Kennedy Institute of Rheumatology Division, Faculty of Medicine, Imperial College of Science Technology and Medicine, London W6 8 LH, United Kingdom
Address correspondence to C. Mauri at her present address, Centre for Rheumatology Research, The Windeyer Institute of Medical Science, 46 Cleveland Street, London W1T 4JF. Phone: 44-207-679-9670; Fax: 44-207-679-9143; E-mail: [email protected]; or M. Londei at his present address, Institute of Child Health, University College London, 30 Guilford Street, London WC1N 1EH, UK. Phone: 44-207-905-2182; Fax: 44-207-905-2316; E-mail: [email protected]
*
Abbreviations used in this paper: CCIA, chronic collagen-induced arthritis; CFSE, carboxyl fluorescein succinimidyl ester; CII, type II collagen; DC, dendritic cell.
Received:
July 30 2002
Revision Received:
December 10 2002
Accepted:
January 03 2003
Online ISSN: 1540-9538
Print ISSN: 0022-1007
The Rockefeller University Press
2003
J Exp Med (2003) 197 (4): 489–501.
Article history
Received:
July 30 2002
Revision Received:
December 10 2002
Accepted:
January 03 2003
Citation
Claudia Mauri, David Gray, Naseem Mushtaq, Marco Londei; Prevention of Arthritis by Interleukin 10–producing B Cells . J Exp Med 17 February 2003; 197 (4): 489–501. doi: https://doi.org/10.1084/jem.20021293
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