We describe here a previously unrecognized property of dendritic cells (DCs), the ability to deacylate the lipid A moiety of gram-negative bacterial LPSs. Both immature DCs of the XS52 cell line and bone marrow–derived DCs produce acyloxyacyl hydrolase, an enzyme that detoxifies LPS by selectively removing the secondary acyl chains from lipid A. Acyloxyacyl hydrolase expression decreased when DCs were incubated with IL-4, IL-1β, TNFα, and an agonistic CD40 antibody (maturation cocktail), and increased after treatment with LPS, CpG oligodeoxynucleotides, or a gram-positive bacterium (Micococcus luteus). Maturation cocktail treatment also diminished, whereas LPS treatment enhanced or maintained the cells' ability to kill Escherichia coli, deacylate LPS, and degrade bacterial protein. Enzymatic deacylation of LPS is an intrinsic, regulated mechanism by which DCs may modulate host responses to this potent bacterial agonist.
Skip Nav Destination
Article navigation
16 June 2003
Article|
June 16 2003
Stimulus-dependent Deacylation of Bacterial Lipopolysaccharide by Dendritic Cells
Mingfang Lu,
Mingfang Lu
1Department of Internal Medicine, University of Texas, Southwestern Medical Center, Dallas, TX 75390
Search for other works by this author on:
Mei Zhang,
Mei Zhang
1Department of Internal Medicine, University of Texas, Southwestern Medical Center, Dallas, TX 75390
Search for other works by this author on:
Richard L. Kitchens,
Richard L. Kitchens
1Department of Internal Medicine, University of Texas, Southwestern Medical Center, Dallas, TX 75390
Search for other works by this author on:
Susan Fosmire,
Susan Fosmire
1Department of Internal Medicine, University of Texas, Southwestern Medical Center, Dallas, TX 75390
Search for other works by this author on:
Akira Takashima,
Akira Takashima
3Department of Dermatology, University of Texas, Southwestern Medical Center, Dallas, TX 75390
Search for other works by this author on:
Robert S. Munford
Robert S. Munford
1Department of Internal Medicine, University of Texas, Southwestern Medical Center, Dallas, TX 75390
2Department of Microbiology, University of Texas, Southwestern Medical Center, Dallas, TX 75390
Search for other works by this author on:
Mingfang Lu
1Department of Internal Medicine, University of Texas, Southwestern Medical Center, Dallas, TX 75390
Mei Zhang
1Department of Internal Medicine, University of Texas, Southwestern Medical Center, Dallas, TX 75390
Richard L. Kitchens
1Department of Internal Medicine, University of Texas, Southwestern Medical Center, Dallas, TX 75390
Susan Fosmire
1Department of Internal Medicine, University of Texas, Southwestern Medical Center, Dallas, TX 75390
Akira Takashima
3Department of Dermatology, University of Texas, Southwestern Medical Center, Dallas, TX 75390
Robert S. Munford
1Department of Internal Medicine, University of Texas, Southwestern Medical Center, Dallas, TX 75390
2Department of Microbiology, University of Texas, Southwestern Medical Center, Dallas, TX 75390
Address correspondence to R.S. Munford, Infectious Disease Division, Dept. of Internal Medicine, University of Texas, Southwestern Medical Center, 5323 Harry Hines Blvd., Dallas, TX 75390-9113. Phone: 214-648-3480; Fax: 214-648-9478; E-mail: [email protected]
*
Abbreviations used in this paper: AOAH, acyloxyacyl hydrolase; BMDC, bone marrow–derived DC; cRPMI, complete RPMI; DC, dendritic cell; ODN, oligodeoxynucleotide; TLR, toll-like receptor.
Received:
March 17 2003
Revision Received:
April 28 2003
Accepted:
April 28 2003
Online ISSN: 1540-9538
Print ISSN: 0022-1007
The Rockefeller University Press
2003
J Exp Med (2003) 197 (12): 1745–1754.
Article history
Received:
March 17 2003
Revision Received:
April 28 2003
Accepted:
April 28 2003
Citation
Mingfang Lu, Mei Zhang, Richard L. Kitchens, Susan Fosmire, Akira Takashima, Robert S. Munford; Stimulus-dependent Deacylation of Bacterial Lipopolysaccharide by Dendritic Cells . J Exp Med 16 June 2003; 197 (12): 1745–1754. doi: https://doi.org/10.1084/jem.20030420
Download citation file:
Sign in
Don't already have an account? Register
Client Account
You could not be signed in. Please check your email address / username and password and try again.
Could not validate captcha. Please try again.
Sign in via your Institution
Sign in via your InstitutionSuggested Content
Email alerts
Advertisement