Chemoattractants and chemokines induce arrest of rolling monocytes during emigration from blood into tissues. In this study, we demonstrated that α4 integrin affinity for vascular cell adhesion molecule (VCAM)-1 was upregulated rapidly and transiently by chemoattractants and stromal cell–derived factor (SDF)-1α and mediated monocyte arrest. α4 integrin affinity changes were detected and blocked using soluble VCAM-1/Fc (sVCAM-1/Fc). In a flow cytometry assay, markedly increased sVCAM-1/Fc binding to human blood monocytes or U937 cells transfected with formyl peptide (FP) receptor was detected 30 s after FP or SDF-1α treatment and declined after 2 min. In a parallel plate flow chamber assay, FP, C5a, platelet-activating factor, or SDF-1α coimmobilized with VCAM-1 induced leukocyte arrest, which was blocked by inclusion of sVCAM-1/Fc but not soluble nonimmune immunoglobulin G in the assay buffer.
Chemoattractants Induce a Rapid and Transient Upregulation of Monocyte α4 Integrin Affinity for Vascular Cell Adhesion Molecule 1 Which Mediates Arrest: An Early Step in the Process of Emigration
Abbreviations used in this paper: CXCR, CXC chemokine receptor; cytD, cytochalasin D; FBS, fetal bovine serum; FP, formyl peptide; ICAM, intercellular adhesion molecule; MCP, monocyte chemotactic protein; PAF, platelet-activating factor; PTx, pertussis toxin; RANTES, regulated upon activation, normal T cell expressed and secreted; SDF, stromal cell–derived factor; VCAM, vascular cell adhesion molecule.
Jason R. Chan, Sharon J. Hyduk, Myron I. Cybulsky; Chemoattractants Induce a Rapid and Transient Upregulation of Monocyte α4 Integrin Affinity for Vascular Cell Adhesion Molecule 1 Which Mediates Arrest: An Early Step in the Process of Emigration. J Exp Med 21 May 2001; 193 (10): 1149–1158. doi: https://doi.org/10.1084/jem.193.10.1149
Download citation file:
Sign in
Client Account
Sign in via your Institution
Sign in via your InstitutionSuggested Content
Email alerts
Advertisement