The T cell antigen receptor (TCR) and pre-TCR complexes are composed of multiple signal-transducing subunits (CD3γ, CD3δ, CD3ε, and ζ) that each contain one or more copies of a semiconserved functional motif, the immunoreceptor tyrosine-based activation motif (ITAM). Although biochemical studies indicate that individual TCR-ITAMs may bind selectively or with different affinity to various effector molecules, data from other experiments suggest that at least some ITAMs are functionally equivalent. In this study, we examined the role of CD3ε ITAM-mediated signals in T cell development by genetically reconstituting CD3ε-deficient mice with transgenes encoding either wild-type or ITAM-mutant (signaling defective) forms of the protein. The results demonstrate that signals transduced by CD3ε are not specifically required for T cell maturation but instead contribute quantitatively to TCR signaling in a manner similar to that previously observed for ζ chain. Unexpectedly, analysis of TCR-transgenic/CD3ε-mutant mice reveals a potential role for CD3ε signals in T cell survival.
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18 September 2000
Brief Definitive Report|
September 18 2000
Function of Cd3ε-Mediated Signals in T Cell Development
Connie L. Sommers,
Connie L. Sommers
aLaboratory of Mammalian Genes and Development, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892
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Jan B. Dejarnette,
Jan B. Dejarnette
aLaboratory of Mammalian Genes and Development, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892
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Kun Huang,
Kun Huang
aLaboratory of Mammalian Genes and Development, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892
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Jan Lee,
Jan Lee
bDivision of Hematologic Products, Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, Maryland 20892
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Dalal El-Khoury,
Dalal El-Khoury
aLaboratory of Mammalian Genes and Development, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892
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Elizabeth W. Shores,
Elizabeth W. Shores
bDivision of Hematologic Products, Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, Maryland 20892
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Paul E. Love
Paul E. Love
aLaboratory of Mammalian Genes and Development, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892
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Connie L. Sommers
aLaboratory of Mammalian Genes and Development, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892
Jan B. Dejarnette
aLaboratory of Mammalian Genes and Development, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892
Kun Huang
aLaboratory of Mammalian Genes and Development, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892
Jan Lee
bDivision of Hematologic Products, Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, Maryland 20892
Dalal El-Khoury
aLaboratory of Mammalian Genes and Development, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892
Elizabeth W. Shores
bDivision of Hematologic Products, Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, Maryland 20892
Paul E. Love
aLaboratory of Mammalian Genes and Development, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892
Connie L. Sommers' present address is Laboratory of Cellular and Molecular Biology, Division of Basic Sciences, National Cancer Institute, Bethesda, MD 20892-4255.
Received:
May 04 2000
Revision Requested:
June 23 2000
Accepted:
July 12 2000
Online ISSN: 1540-9538
Print ISSN: 0022-1007
© 2000 The Rockefeller University Press
2000
The Rockefeller University Press
J Exp Med (2000) 192 (6): 913–920.
Article history
Received:
May 04 2000
Revision Requested:
June 23 2000
Accepted:
July 12 2000
Citation
Connie L. Sommers, Jan B. Dejarnette, Kun Huang, Jan Lee, Dalal El-Khoury, Elizabeth W. Shores, Paul E. Love; Function of Cd3ε-Mediated Signals in T Cell Development. J Exp Med 18 September 2000; 192 (6): 913–920. doi: https://doi.org/10.1084/jem.192.6.913
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