The developmental requirements for immunological memory, a central feature of adaptive immune responses, is largely obscure. We show that as naive CD8 T cells undergo homeostasis-driven proliferation in lymphopenic mice in the absence of overt antigenic stimulation, they progressively acquire phenotypic and functional characteristics of antigen-induced memory CD8 T cells. Thus, the homeostasis-induced memory CD8 T cells express typical memory cell markers, lyse target cells directly in vitro and in vivo, respond to lower doses of antigen than naive cells, and secrete interferon γ faster upon restimulation. Like antigen-induced memory T cell differentiation, the homeostasis-driven process requires T cell proliferation and, initially, the presence of appropriate restricting major histocompatibility complexes, but it differs by occurring without effector cell formation and without requiring interleukin 2 or costimulation via CD28. These findings define repetitive cell division plus T cell receptor ligation as the basic requirements for naive to memory T cell differentiation.
Skip Nav Destination
Article navigation
21 August 2000
Article|
August 21 2000
Homeostasis-Stimulated Proliferation Drives Naive T Cells to Differentiate Directly into Memory T Cells
Bryan K. Cho,
Bryan K. Cho
aCenter for Cancer Research and Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139
Search for other works by this author on:
Varada P. Rao,
Varada P. Rao
aCenter for Cancer Research and Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139
Search for other works by this author on:
Qing Ge,
Qing Ge
aCenter for Cancer Research and Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139
Search for other works by this author on:
Herman N. Eisen,
Herman N. Eisen
aCenter for Cancer Research and Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139
Search for other works by this author on:
Jianzhu Chen
Jianzhu Chen
aCenter for Cancer Research and Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139
Search for other works by this author on:
Bryan K. Cho
aCenter for Cancer Research and Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139
Varada P. Rao
aCenter for Cancer Research and Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139
Qing Ge
aCenter for Cancer Research and Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139
Herman N. Eisen
aCenter for Cancer Research and Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139
Jianzhu Chen
aCenter for Cancer Research and Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139
Abbreviations used in this paper: B6, C57BL/6; CFSE, carboxyfluorescein diacetate-succinimidyl ester; GFP, green fluorescent protein; RAG, recombination activating gene.
B.K. Cho's present address is Medical Scholars Program and Department of Biochemistry, University of Illinois, Urbana, IL 61801.
Received:
February 29 2000
Revision Requested:
May 04 2000
Accepted:
June 06 2000
Online ISSN: 1540-9538
Print ISSN: 0022-1007
© 2000 The Rockefeller University Press
2000
The Rockefeller University Press
J Exp Med (2000) 192 (4): 549–556.
Article history
Received:
February 29 2000
Revision Requested:
May 04 2000
Accepted:
June 06 2000
Citation
Bryan K. Cho, Varada P. Rao, Qing Ge, Herman N. Eisen, Jianzhu Chen; Homeostasis-Stimulated Proliferation Drives Naive T Cells to Differentiate Directly into Memory T Cells. J Exp Med 21 August 2000; 192 (4): 549–556. doi: https://doi.org/10.1084/jem.192.4.549
Download citation file:
Sign in
Don't already have an account? Register
Client Account
You could not be signed in. Please check your email address / username and password and try again.
Could not validate captcha. Please try again.
Sign in via your Institution
Sign in via your InstitutionSuggested Content
Email alerts
Advertisement