The many binding studies of monoclonal immunoglobulin (Ig) produced by plasmacytomas have found no universally common binding properties, but instead, groups of plasmacytomas with specific antigen-binding activities to haptens such as phosphorylcholine, dextrans, fructofuranans, or dinitrophenyl. Subsequently, it was found that plasmacytomas with similar binding chain specificities not only expressed the same idiotype, but rearranged the same light (VL) and heavy (VH) variable region genes to express a characteristic monoclonal antibody. In this study, we have examined by enzyme-linked immunosorbent assay five antibodies secreted by silicone-induced mouse plasmacytomas using a broader panel of antigens including actin, myosin, tubulin, single-stranded DNA, and double-stranded DNA. We have determined the Ig heavy and light chain V gene usage in these same plasmacytomas at the DNA and RNA level. Our studies reveal: (a) antibodies secreted by plasmacytomas bind to different antigens in a manner similar to that observed for natural autoantibodies; (b) the expressed Ig heavy genes are restricted in V gene usage to the VH-J558 family; and (c) secondary rearrangements occur at the light chain level with at least three plasmacytomas expressing both κ and λ light chain genes. These results suggest that plasmacytomas use a restricted population of B cells that may still be undergoing rearrangement, thereby bypassing the allelic exclusion normally associated with expression of antibody genes.
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15 November 1999
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November 15 1999
Restricted Immunoglobulin Variable Region (Ig V) Gene Expression Accompanies Secondary Rearrangements of Light Chain Ig V Genes in Mouse Plasmacytomas
Lena Diaw,
Lena Diaw
aLaboratory of Genetics, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892
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David Siwarski,
David Siwarski
aLaboratory of Genetics, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892
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Allen Coleman,
Allen Coleman
aLaboratory of Genetics, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892
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Jennifer Kim,
Jennifer Kim
aLaboratory of Genetics, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892
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Gary M. Jones,
Gary M. Jones
aLaboratory of Genetics, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892
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Guillaume Dighiero,
Guillaume Dighiero
bLaboratoire d'Immunohematologie et Immunopathologie, Institut Pasteur, 75724 Paris Cedex 15, France
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Konrad Huppi
Konrad Huppi
aLaboratory of Genetics, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892
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Lena Diaw
aLaboratory of Genetics, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892
David Siwarski
aLaboratory of Genetics, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892
Allen Coleman
aLaboratory of Genetics, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892
Jennifer Kim
aLaboratory of Genetics, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892
Gary M. Jones
aLaboratory of Genetics, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892
Guillaume Dighiero
bLaboratoire d'Immunohematologie et Immunopathologie, Institut Pasteur, 75724 Paris Cedex 15, France
Konrad Huppi
aLaboratory of Genetics, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892
1used in this paper: CLL, chronic lymphocytic leukemia; ds, double-stranded; g, generation; GC, germinal center; IVS, intervening sequence; NAA, natural polyreactive autoantibody; PC, plasmacytoma; R, replacement; RAG, recombination activating gene; RT, reverse transcription; S, silent; SI, silicone-induced PC; ss, single-stranded
Received:
June 02 1999
Revision Requested:
September 09 1999
Accepted:
September 13 1999
Online ISSN: 1540-9538
Print ISSN: 0022-1007
© 1999 The Rockefeller University Press
1999
The Rockefeller University Press
J Exp Med (1999) 190 (10): 1405–1416.
Article history
Received:
June 02 1999
Revision Requested:
September 09 1999
Accepted:
September 13 1999
Citation
Lena Diaw, David Siwarski, Allen Coleman, Jennifer Kim, Gary M. Jones, Guillaume Dighiero, Konrad Huppi; Restricted Immunoglobulin Variable Region (Ig V) Gene Expression Accompanies Secondary Rearrangements of Light Chain Ig V Genes in Mouse Plasmacytomas. J Exp Med 15 November 1999; 190 (10): 1405–1416. doi: https://doi.org/10.1084/jem.190.10.1405
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