The goal of this study was to determine the mechanisms by which dendritic cells (DCs) in blood could interact with endothelium, a prerequisite to extravasation into tissues. Our results indicate that DCs express both HECA-452–reactive and nonreactive isoforms of P-selectin glycoprotein ligand 1 (PSGL-1) and can tether and roll efficiently on E- and P-selectin under flow conditions in vitro. Freshly isolated blood DCs were further observed to roll continuously along noninflamed murine dermal endothelium in vivo. This interaction is strictly dependent on endothelial selectins, as shown by experiments with blocking antibodies and with E- and P-selectin–deficient mice. We hypothesize that DCs in blood are constitutively poised at the interface of blood and skin, ready to extravasate upon induction of inflammation, and we showed that cutaneous inflammation results in a rapid recruitment of DCs from the blood to tissues. We propose that this is an important and previously unappreciated element of immunosurveillance.
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15 February 1999
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February 15 1999
Interaction of Dendritic Cells with Skin Endothelium: A New Perspective on Immunosurveillance
Caroline Robert,
Caroline Robert
From the *Harvard Skin Disease Research Center, Division of Dermatology, Brigham and Women's Hospital, Harvard Institutes of Medicine, Boston, Massachusetts 02115; ‡MatTek Corporation, Ashland, Massachusetts 01721; the §Center for Cancer Research, Department of Biology, Howard Hughes Medical Institute, Massachusetts Institute of Technology, Cambridge, Massachusetts 01239; and the ‖Center for Blood Research, Harvard Medical School, Boston, Massachusetts 02115
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Robert C. Fuhlbrigge,
Robert C. Fuhlbrigge
From the *Harvard Skin Disease Research Center, Division of Dermatology, Brigham and Women's Hospital, Harvard Institutes of Medicine, Boston, Massachusetts 02115; ‡MatTek Corporation, Ashland, Massachusetts 01721; the §Center for Cancer Research, Department of Biology, Howard Hughes Medical Institute, Massachusetts Institute of Technology, Cambridge, Massachusetts 01239; and the ‖Center for Blood Research, Harvard Medical School, Boston, Massachusetts 02115
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J. David Kieffer,
J. David Kieffer
From the *Harvard Skin Disease Research Center, Division of Dermatology, Brigham and Women's Hospital, Harvard Institutes of Medicine, Boston, Massachusetts 02115; ‡MatTek Corporation, Ashland, Massachusetts 01721; the §Center for Cancer Research, Department of Biology, Howard Hughes Medical Institute, Massachusetts Institute of Technology, Cambridge, Massachusetts 01239; and the ‖Center for Blood Research, Harvard Medical School, Boston, Massachusetts 02115
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Seyoum Ayehunie,
Seyoum Ayehunie
From the *Harvard Skin Disease Research Center, Division of Dermatology, Brigham and Women's Hospital, Harvard Institutes of Medicine, Boston, Massachusetts 02115; ‡MatTek Corporation, Ashland, Massachusetts 01721; the §Center for Cancer Research, Department of Biology, Howard Hughes Medical Institute, Massachusetts Institute of Technology, Cambridge, Massachusetts 01239; and the ‖Center for Blood Research, Harvard Medical School, Boston, Massachusetts 02115
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Richard O. Hynes,
Richard O. Hynes
From the *Harvard Skin Disease Research Center, Division of Dermatology, Brigham and Women's Hospital, Harvard Institutes of Medicine, Boston, Massachusetts 02115; ‡MatTek Corporation, Ashland, Massachusetts 01721; the §Center for Cancer Research, Department of Biology, Howard Hughes Medical Institute, Massachusetts Institute of Technology, Cambridge, Massachusetts 01239; and the ‖Center for Blood Research, Harvard Medical School, Boston, Massachusetts 02115
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Guiying Cheng,
Guiying Cheng
From the *Harvard Skin Disease Research Center, Division of Dermatology, Brigham and Women's Hospital, Harvard Institutes of Medicine, Boston, Massachusetts 02115; ‡MatTek Corporation, Ashland, Massachusetts 01721; the §Center for Cancer Research, Department of Biology, Howard Hughes Medical Institute, Massachusetts Institute of Technology, Cambridge, Massachusetts 01239; and the ‖Center for Blood Research, Harvard Medical School, Boston, Massachusetts 02115
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Stephan Grabbe,
Stephan Grabbe
From the *Harvard Skin Disease Research Center, Division of Dermatology, Brigham and Women's Hospital, Harvard Institutes of Medicine, Boston, Massachusetts 02115; ‡MatTek Corporation, Ashland, Massachusetts 01721; the §Center for Cancer Research, Department of Biology, Howard Hughes Medical Institute, Massachusetts Institute of Technology, Cambridge, Massachusetts 01239; and the ‖Center for Blood Research, Harvard Medical School, Boston, Massachusetts 02115
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Ulrich H. von Andrian,
Ulrich H. von Andrian
From the *Harvard Skin Disease Research Center, Division of Dermatology, Brigham and Women's Hospital, Harvard Institutes of Medicine, Boston, Massachusetts 02115; ‡MatTek Corporation, Ashland, Massachusetts 01721; the §Center for Cancer Research, Department of Biology, Howard Hughes Medical Institute, Massachusetts Institute of Technology, Cambridge, Massachusetts 01239; and the ‖Center for Blood Research, Harvard Medical School, Boston, Massachusetts 02115
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Thomas S. Kupper
Thomas S. Kupper
From the *Harvard Skin Disease Research Center, Division of Dermatology, Brigham and Women's Hospital, Harvard Institutes of Medicine, Boston, Massachusetts 02115; ‡MatTek Corporation, Ashland, Massachusetts 01721; the §Center for Cancer Research, Department of Biology, Howard Hughes Medical Institute, Massachusetts Institute of Technology, Cambridge, Massachusetts 01239; and the ‖Center for Blood Research, Harvard Medical School, Boston, Massachusetts 02115
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Caroline Robert
From the *Harvard Skin Disease Research Center, Division of Dermatology, Brigham and Women's Hospital, Harvard Institutes of Medicine, Boston, Massachusetts 02115; ‡MatTek Corporation, Ashland, Massachusetts 01721; the §Center for Cancer Research, Department of Biology, Howard Hughes Medical Institute, Massachusetts Institute of Technology, Cambridge, Massachusetts 01239; and the ‖Center for Blood Research, Harvard Medical School, Boston, Massachusetts 02115
Robert C. Fuhlbrigge
From the *Harvard Skin Disease Research Center, Division of Dermatology, Brigham and Women's Hospital, Harvard Institutes of Medicine, Boston, Massachusetts 02115; ‡MatTek Corporation, Ashland, Massachusetts 01721; the §Center for Cancer Research, Department of Biology, Howard Hughes Medical Institute, Massachusetts Institute of Technology, Cambridge, Massachusetts 01239; and the ‖Center for Blood Research, Harvard Medical School, Boston, Massachusetts 02115
J. David Kieffer
From the *Harvard Skin Disease Research Center, Division of Dermatology, Brigham and Women's Hospital, Harvard Institutes of Medicine, Boston, Massachusetts 02115; ‡MatTek Corporation, Ashland, Massachusetts 01721; the §Center for Cancer Research, Department of Biology, Howard Hughes Medical Institute, Massachusetts Institute of Technology, Cambridge, Massachusetts 01239; and the ‖Center for Blood Research, Harvard Medical School, Boston, Massachusetts 02115
Seyoum Ayehunie
From the *Harvard Skin Disease Research Center, Division of Dermatology, Brigham and Women's Hospital, Harvard Institutes of Medicine, Boston, Massachusetts 02115; ‡MatTek Corporation, Ashland, Massachusetts 01721; the §Center for Cancer Research, Department of Biology, Howard Hughes Medical Institute, Massachusetts Institute of Technology, Cambridge, Massachusetts 01239; and the ‖Center for Blood Research, Harvard Medical School, Boston, Massachusetts 02115
Richard O. Hynes
From the *Harvard Skin Disease Research Center, Division of Dermatology, Brigham and Women's Hospital, Harvard Institutes of Medicine, Boston, Massachusetts 02115; ‡MatTek Corporation, Ashland, Massachusetts 01721; the §Center for Cancer Research, Department of Biology, Howard Hughes Medical Institute, Massachusetts Institute of Technology, Cambridge, Massachusetts 01239; and the ‖Center for Blood Research, Harvard Medical School, Boston, Massachusetts 02115
Guiying Cheng
From the *Harvard Skin Disease Research Center, Division of Dermatology, Brigham and Women's Hospital, Harvard Institutes of Medicine, Boston, Massachusetts 02115; ‡MatTek Corporation, Ashland, Massachusetts 01721; the §Center for Cancer Research, Department of Biology, Howard Hughes Medical Institute, Massachusetts Institute of Technology, Cambridge, Massachusetts 01239; and the ‖Center for Blood Research, Harvard Medical School, Boston, Massachusetts 02115
Stephan Grabbe
From the *Harvard Skin Disease Research Center, Division of Dermatology, Brigham and Women's Hospital, Harvard Institutes of Medicine, Boston, Massachusetts 02115; ‡MatTek Corporation, Ashland, Massachusetts 01721; the §Center for Cancer Research, Department of Biology, Howard Hughes Medical Institute, Massachusetts Institute of Technology, Cambridge, Massachusetts 01239; and the ‖Center for Blood Research, Harvard Medical School, Boston, Massachusetts 02115
Ulrich H. von Andrian
From the *Harvard Skin Disease Research Center, Division of Dermatology, Brigham and Women's Hospital, Harvard Institutes of Medicine, Boston, Massachusetts 02115; ‡MatTek Corporation, Ashland, Massachusetts 01721; the §Center for Cancer Research, Department of Biology, Howard Hughes Medical Institute, Massachusetts Institute of Technology, Cambridge, Massachusetts 01239; and the ‖Center for Blood Research, Harvard Medical School, Boston, Massachusetts 02115
Thomas S. Kupper
From the *Harvard Skin Disease Research Center, Division of Dermatology, Brigham and Women's Hospital, Harvard Institutes of Medicine, Boston, Massachusetts 02115; ‡MatTek Corporation, Ashland, Massachusetts 01721; the §Center for Cancer Research, Department of Biology, Howard Hughes Medical Institute, Massachusetts Institute of Technology, Cambridge, Massachusetts 01239; and the ‖Center for Blood Research, Harvard Medical School, Boston, Massachusetts 02115
Address correspondence to Thomas S. Kupper, Harvard Skin Disease Research Center, Division of Dermatology, Department of Medicine, Brigham and Women's Hospital, Harvard Institutes of Medicine, 77 Avenue Louis Pasteur, Boston, MA 02115. Phone: 617-525-5550; Fax: 617-525-5571; E-mail: [email protected]
Received:
October 13 1998
Revision Received:
December 29 1998
Online ISSN: 1540-9538
Print ISSN: 0022-1007
1999
J Exp Med (1999) 189 (4): 627–636.
Article history
Received:
October 13 1998
Revision Received:
December 29 1998
Citation
Caroline Robert, Robert C. Fuhlbrigge, J. David Kieffer, Seyoum Ayehunie, Richard O. Hynes, Guiying Cheng, Stephan Grabbe, Ulrich H. von Andrian, Thomas S. Kupper; Interaction of Dendritic Cells with Skin Endothelium: A New Perspective on Immunosurveillance . J Exp Med 15 February 1999; 189 (4): 627–636. doi: https://doi.org/10.1084/jem.189.4.627
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