Previous studies in murine models, including those using the β2 microglobulin knockout mouse, have suggested an important role for CD8+ T cells in host defense to Mycobacterium tuberculosis (Mtb). At present, little is understood about these cells in the human immune response to tuberculosis. This report demonstrates the existence of human Mtb-reactive CD8+ T cells. These cells are present preferentially in persons infected with Mtb and produce interferon γ in response to stimulation with Mtb-infected target cells. Recognition of Mtb-infected cells by these CD8+ T cells is restricted neither by the major histocompatibility complex (MHC) class I A, B, or C alleles nor by CD1, although it is inhibited by anti–MHC class I antibody. The Mtb-specific CD8+ T cells recognize an antigen which is generated in the proteasome, but which does not require transport through the Golgi-ER. The data suggest the possible use of nonpolymorphic MHC class Ib antigen presenting structures other than CD1.
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18 May 1998
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May 18 1998
Characterization of Human CD8+ T Cells Reactive with Mycobacterium tuberculosis–infected Antigen-presenting Cells
David M. Lewinsohn,
David M. Lewinsohn
From the *Division of Pulmonary & Critical Care Medicine, University of Washington, Seattle, Washington 98104; the ‡Infectious Disease Research Institute, Seattle, Washington 98104; the §Corixa Corporation, Seattle, Washington 98104; and the ‖Fred Hutchinson Cancer Research Center, Seattle, Washington 98104
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Mark R. Alderson,
Mark R. Alderson
From the *Division of Pulmonary & Critical Care Medicine, University of Washington, Seattle, Washington 98104; the ‡Infectious Disease Research Institute, Seattle, Washington 98104; the §Corixa Corporation, Seattle, Washington 98104; and the ‖Fred Hutchinson Cancer Research Center, Seattle, Washington 98104
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Andria L. Briden,
Andria L. Briden
From the *Division of Pulmonary & Critical Care Medicine, University of Washington, Seattle, Washington 98104; the ‡Infectious Disease Research Institute, Seattle, Washington 98104; the §Corixa Corporation, Seattle, Washington 98104; and the ‖Fred Hutchinson Cancer Research Center, Seattle, Washington 98104
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Stanley R. Riddell,
Stanley R. Riddell
From the *Division of Pulmonary & Critical Care Medicine, University of Washington, Seattle, Washington 98104; the ‡Infectious Disease Research Institute, Seattle, Washington 98104; the §Corixa Corporation, Seattle, Washington 98104; and the ‖Fred Hutchinson Cancer Research Center, Seattle, Washington 98104
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Steven G. Reed,
Steven G. Reed
From the *Division of Pulmonary & Critical Care Medicine, University of Washington, Seattle, Washington 98104; the ‡Infectious Disease Research Institute, Seattle, Washington 98104; the §Corixa Corporation, Seattle, Washington 98104; and the ‖Fred Hutchinson Cancer Research Center, Seattle, Washington 98104
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Kenneth H. Grabstein
Kenneth H. Grabstein
From the *Division of Pulmonary & Critical Care Medicine, University of Washington, Seattle, Washington 98104; the ‡Infectious Disease Research Institute, Seattle, Washington 98104; the §Corixa Corporation, Seattle, Washington 98104; and the ‖Fred Hutchinson Cancer Research Center, Seattle, Washington 98104
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David M. Lewinsohn
From the *Division of Pulmonary & Critical Care Medicine, University of Washington, Seattle, Washington 98104; the ‡Infectious Disease Research Institute, Seattle, Washington 98104; the §Corixa Corporation, Seattle, Washington 98104; and the ‖Fred Hutchinson Cancer Research Center, Seattle, Washington 98104
Mark R. Alderson
From the *Division of Pulmonary & Critical Care Medicine, University of Washington, Seattle, Washington 98104; the ‡Infectious Disease Research Institute, Seattle, Washington 98104; the §Corixa Corporation, Seattle, Washington 98104; and the ‖Fred Hutchinson Cancer Research Center, Seattle, Washington 98104
Andria L. Briden
From the *Division of Pulmonary & Critical Care Medicine, University of Washington, Seattle, Washington 98104; the ‡Infectious Disease Research Institute, Seattle, Washington 98104; the §Corixa Corporation, Seattle, Washington 98104; and the ‖Fred Hutchinson Cancer Research Center, Seattle, Washington 98104
Stanley R. Riddell
From the *Division of Pulmonary & Critical Care Medicine, University of Washington, Seattle, Washington 98104; the ‡Infectious Disease Research Institute, Seattle, Washington 98104; the §Corixa Corporation, Seattle, Washington 98104; and the ‖Fred Hutchinson Cancer Research Center, Seattle, Washington 98104
Steven G. Reed
From the *Division of Pulmonary & Critical Care Medicine, University of Washington, Seattle, Washington 98104; the ‡Infectious Disease Research Institute, Seattle, Washington 98104; the §Corixa Corporation, Seattle, Washington 98104; and the ‖Fred Hutchinson Cancer Research Center, Seattle, Washington 98104
Kenneth H. Grabstein
From the *Division of Pulmonary & Critical Care Medicine, University of Washington, Seattle, Washington 98104; the ‡Infectious Disease Research Institute, Seattle, Washington 98104; the §Corixa Corporation, Seattle, Washington 98104; and the ‖Fred Hutchinson Cancer Research Center, Seattle, Washington 98104
Address correspondence to David M. Lewinsohn, Infectious Disease Research Institute, Box 358080, 1124 Columbia St., Seattle, Washington 98104. Phone: 206-754-5755; Fax: 206-754-5715; E-mail: [email protected]
This work was presented in part at the ASM Conference “Tuberculosis: Past, Present, and Future”, Copper Mountain, CO, July 8–12, 1997.
Received:
September 17 1997
Revision Received:
March 19 1998
Online ISSN: 1540-9538
Print ISSN: 0022-1007
1998
J Exp Med (1998) 187 (10): 1633–1640.
Article history
Received:
September 17 1997
Revision Received:
March 19 1998
Citation
David M. Lewinsohn, Mark R. Alderson, Andria L. Briden, Stanley R. Riddell, Steven G. Reed, Kenneth H. Grabstein; Characterization of Human CD8+ T Cells Reactive with Mycobacterium tuberculosis–infected Antigen-presenting Cells . J Exp Med 18 May 1998; 187 (10): 1633–1640. doi: https://doi.org/10.1084/jem.187.10.1633
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