Macrophage tropic HIV-1 is predominant during the initial viremia after person to person transmission of HIV-1 (Zhu, T., H. Mo, N. Wang, D.S. Nam, Y. Cao, R.A. Koup, and D.D. Ho. 1993. Science. 261:1179–1181.), and this selection may occur during virus entry and carriage to the lymphoid tissue. Human skin explants were used to model HIV-1 selection that may occur at the skin or mucosal surface. Macrophage tropic, but not T cell line tropic strains of HIV-1 applied to the abraded epidermis were recovered from the cells emigrating from the skin explants. Dermis and epidermis were separated by dispase digestion after virus exposure to determine the site of viral selection within the skin. Uptake and transmission to T cells of all HIV-1 isolates was found with the dermal emigrant cells, but only macrophage tropic virus was transferred by emigrants from the epidermis exposed to HIV-1, indicating selection only within the epidermis. CD3+, CD4+ T cells were found in both the dermal and epidermal emigrant cells. After cell sorting to exclude contaminating T cells, macrophage tropic HIV-1 was found in both the dermal emigrant dendritic cells and in dendritic cells sorted from the epidermal emigrants. These observations suggest that selective infection of the immature epidermal dendritic cells represents the cellular mechanism that limits the initial viremia to HIV-1 that can use the CCR5 coreceptor.
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18 May 1998
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May 18 1998
HIV-1 Selection by Epidermal Dendritic Cells during Transmission across Human Skin
Jeanette C. Reece,
Jeanette C. Reece
From the *Department of Microbiology and Immunology, University of Melbourne, Parkville, Victoria, Australia 3052; the ‡Alfred Hospital, Prahran, Victoria, Australia 3181; the §Department of Plastic Surgery, St. Vincent's Hospital, Melbourne, Victoria, Australia 3065; and the ‖AIDS Pathogenesis Research Unit, Macfarlane Burnet Centre for Medical Research, Fairfield, Victoria, Australia 3078
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Amanda J. Handley,
Amanda J. Handley
From the *Department of Microbiology and Immunology, University of Melbourne, Parkville, Victoria, Australia 3052; the ‡Alfred Hospital, Prahran, Victoria, Australia 3181; the §Department of Plastic Surgery, St. Vincent's Hospital, Melbourne, Victoria, Australia 3065; and the ‖AIDS Pathogenesis Research Unit, Macfarlane Burnet Centre for Medical Research, Fairfield, Victoria, Australia 3078
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E. John Anstee,
E. John Anstee
From the *Department of Microbiology and Immunology, University of Melbourne, Parkville, Victoria, Australia 3052; the ‡Alfred Hospital, Prahran, Victoria, Australia 3181; the §Department of Plastic Surgery, St. Vincent's Hospital, Melbourne, Victoria, Australia 3065; and the ‖AIDS Pathogenesis Research Unit, Macfarlane Burnet Centre for Medical Research, Fairfield, Victoria, Australia 3078
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Wayne A. Morrison,
Wayne A. Morrison
From the *Department of Microbiology and Immunology, University of Melbourne, Parkville, Victoria, Australia 3052; the ‡Alfred Hospital, Prahran, Victoria, Australia 3181; the §Department of Plastic Surgery, St. Vincent's Hospital, Melbourne, Victoria, Australia 3065; and the ‖AIDS Pathogenesis Research Unit, Macfarlane Burnet Centre for Medical Research, Fairfield, Victoria, Australia 3078
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Suzanne M. Crowe,
Suzanne M. Crowe
From the *Department of Microbiology and Immunology, University of Melbourne, Parkville, Victoria, Australia 3052; the ‡Alfred Hospital, Prahran, Victoria, Australia 3181; the §Department of Plastic Surgery, St. Vincent's Hospital, Melbourne, Victoria, Australia 3065; and the ‖AIDS Pathogenesis Research Unit, Macfarlane Burnet Centre for Medical Research, Fairfield, Victoria, Australia 3078
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Paul U. Cameron
Paul U. Cameron
From the *Department of Microbiology and Immunology, University of Melbourne, Parkville, Victoria, Australia 3052; the ‡Alfred Hospital, Prahran, Victoria, Australia 3181; the §Department of Plastic Surgery, St. Vincent's Hospital, Melbourne, Victoria, Australia 3065; and the ‖AIDS Pathogenesis Research Unit, Macfarlane Burnet Centre for Medical Research, Fairfield, Victoria, Australia 3078
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Jeanette C. Reece
From the *Department of Microbiology and Immunology, University of Melbourne, Parkville, Victoria, Australia 3052; the ‡Alfred Hospital, Prahran, Victoria, Australia 3181; the §Department of Plastic Surgery, St. Vincent's Hospital, Melbourne, Victoria, Australia 3065; and the ‖AIDS Pathogenesis Research Unit, Macfarlane Burnet Centre for Medical Research, Fairfield, Victoria, Australia 3078
Amanda J. Handley
From the *Department of Microbiology and Immunology, University of Melbourne, Parkville, Victoria, Australia 3052; the ‡Alfred Hospital, Prahran, Victoria, Australia 3181; the §Department of Plastic Surgery, St. Vincent's Hospital, Melbourne, Victoria, Australia 3065; and the ‖AIDS Pathogenesis Research Unit, Macfarlane Burnet Centre for Medical Research, Fairfield, Victoria, Australia 3078
E. John Anstee
From the *Department of Microbiology and Immunology, University of Melbourne, Parkville, Victoria, Australia 3052; the ‡Alfred Hospital, Prahran, Victoria, Australia 3181; the §Department of Plastic Surgery, St. Vincent's Hospital, Melbourne, Victoria, Australia 3065; and the ‖AIDS Pathogenesis Research Unit, Macfarlane Burnet Centre for Medical Research, Fairfield, Victoria, Australia 3078
Wayne A. Morrison
From the *Department of Microbiology and Immunology, University of Melbourne, Parkville, Victoria, Australia 3052; the ‡Alfred Hospital, Prahran, Victoria, Australia 3181; the §Department of Plastic Surgery, St. Vincent's Hospital, Melbourne, Victoria, Australia 3065; and the ‖AIDS Pathogenesis Research Unit, Macfarlane Burnet Centre for Medical Research, Fairfield, Victoria, Australia 3078
Suzanne M. Crowe
From the *Department of Microbiology and Immunology, University of Melbourne, Parkville, Victoria, Australia 3052; the ‡Alfred Hospital, Prahran, Victoria, Australia 3181; the §Department of Plastic Surgery, St. Vincent's Hospital, Melbourne, Victoria, Australia 3065; and the ‖AIDS Pathogenesis Research Unit, Macfarlane Burnet Centre for Medical Research, Fairfield, Victoria, Australia 3078
Paul U. Cameron
From the *Department of Microbiology and Immunology, University of Melbourne, Parkville, Victoria, Australia 3052; the ‡Alfred Hospital, Prahran, Victoria, Australia 3181; the §Department of Plastic Surgery, St. Vincent's Hospital, Melbourne, Victoria, Australia 3065; and the ‖AIDS Pathogenesis Research Unit, Macfarlane Burnet Centre for Medical Research, Fairfield, Victoria, Australia 3078
Address correspondence to Paul U. Cameron, Department of Microbiology and Immunology, University of Melbourne, Grattan St., Parkville, Victoria, Australia 3052. Phone: 61-3-9344-5688; Fax: 61-3-9347-1540; E-mail: [email protected]
Received:
June 26 1997
Revision Received:
February 09 1998
Online ISSN: 1540-9538
Print ISSN: 0022-1007
1998
J Exp Med (1998) 187 (10): 1623–1631.
Article history
Received:
June 26 1997
Revision Received:
February 09 1998
Citation
Jeanette C. Reece, Amanda J. Handley, E. John Anstee, Wayne A. Morrison, Suzanne M. Crowe, Paul U. Cameron; HIV-1 Selection by Epidermal Dendritic Cells during Transmission across Human Skin . J Exp Med 18 May 1998; 187 (10): 1623–1631. doi: https://doi.org/10.1084/jem.187.10.1623
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