We isolated a new mouse gene that is highly expressed in thymocytes, testis, and brain. This gene, SRG3, showed a significant sequence homology to SWI3, a yeast transcriptional activator, and its human homolog BAF155. SRG3 encodes 1,100 amino acids and has 33–47% identity with SWI3 protein over three regions. The SRG3 protein contains an acidic NH2 terminus, a myb-like DNA binding domain, a leucine-zipper motif, and a proline- and glutamine-rich region at its COOH terminus. Rabbit antiserum raised against a COOH-terminal polypeptide of the SRG3 recognized a protein with an apparent molecular mass of 155 kD. The serum also detected a 170-kD protein that seems to be a mouse homologue of human BAF170. Immunoprecipitation of cell extract with the antiserum against the mouse SRG3 also brought down a 195-kD protein that could be recognized by an antiserum raised against human SWI2 protein. The results suggest that the SRG3 protein associates with a mouse SWI2. The SRG3 protein is expressed about three times higher in thymocytes than in peripheral lymphocytes. The expression of anti-sense RNA to SRG3 mRNA in a thymoma cell line, S49.1, reduced the expression level of the SRG3 protein, and decreased the apoptotic cell death induced by glucocorticoids. These results suggest that the SRG3 protein is involved in the glucocorticoid-induced apoptosis in the thymoma cell line. This implicates that the SRG3 may play an important regulatory role during T cell development in thymus.
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19 May 1997
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May 19 1997
A New Mouse Gene, SRG3, Related to the SWI3 of Saccharomyces cerevisiae, Is Required for Apoptosis Induced by Glucocorticoids in a Thymoma Cell Line
Sung H. Jeon,
Sung H. Jeon
From the *Institute for Molecular Biology and Genetics and Department of Molecular Biology, Seoul National University, Seoul 151-742, Korea; the ‡Laboratory of Immunology, Korea Cancer Center Hospital, Seoul 139-240, Korea; and the §Department of Molecular Biology, Dankook University, Seoul 140-714, Korea
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Myeong G. Kang,
Myeong G. Kang
From the *Institute for Molecular Biology and Genetics and Department of Molecular Biology, Seoul National University, Seoul 151-742, Korea; the ‡Laboratory of Immunology, Korea Cancer Center Hospital, Seoul 139-240, Korea; and the §Department of Molecular Biology, Dankook University, Seoul 140-714, Korea
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Young H. Kim,
Young H. Kim
From the *Institute for Molecular Biology and Genetics and Department of Molecular Biology, Seoul National University, Seoul 151-742, Korea; the ‡Laboratory of Immunology, Korea Cancer Center Hospital, Seoul 139-240, Korea; and the §Department of Molecular Biology, Dankook University, Seoul 140-714, Korea
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Yong H. Jin,
Yong H. Jin
From the *Institute for Molecular Biology and Genetics and Department of Molecular Biology, Seoul National University, Seoul 151-742, Korea; the ‡Laboratory of Immunology, Korea Cancer Center Hospital, Seoul 139-240, Korea; and the §Department of Molecular Biology, Dankook University, Seoul 140-714, Korea
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Changjin Lee,
Changjin Lee
From the *Institute for Molecular Biology and Genetics and Department of Molecular Biology, Seoul National University, Seoul 151-742, Korea; the ‡Laboratory of Immunology, Korea Cancer Center Hospital, Seoul 139-240, Korea; and the §Department of Molecular Biology, Dankook University, Seoul 140-714, Korea
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Hee-Yong Chung,
Hee-Yong Chung
From the *Institute for Molecular Biology and Genetics and Department of Molecular Biology, Seoul National University, Seoul 151-742, Korea; the ‡Laboratory of Immunology, Korea Cancer Center Hospital, Seoul 139-240, Korea; and the §Department of Molecular Biology, Dankook University, Seoul 140-714, Korea
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Hyockman Kwon,
Hyockman Kwon
From the *Institute for Molecular Biology and Genetics and Department of Molecular Biology, Seoul National University, Seoul 151-742, Korea; the ‡Laboratory of Immunology, Korea Cancer Center Hospital, Seoul 139-240, Korea; and the §Department of Molecular Biology, Dankook University, Seoul 140-714, Korea
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Sang D. Park,
Sang D. Park
From the *Institute for Molecular Biology and Genetics and Department of Molecular Biology, Seoul National University, Seoul 151-742, Korea; the ‡Laboratory of Immunology, Korea Cancer Center Hospital, Seoul 139-240, Korea; and the §Department of Molecular Biology, Dankook University, Seoul 140-714, Korea
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Rho H. Seong
Rho H. Seong
From the *Institute for Molecular Biology and Genetics and Department of Molecular Biology, Seoul National University, Seoul 151-742, Korea; the ‡Laboratory of Immunology, Korea Cancer Center Hospital, Seoul 139-240, Korea; and the §Department of Molecular Biology, Dankook University, Seoul 140-714, Korea
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Sung H. Jeon
From the *Institute for Molecular Biology and Genetics and Department of Molecular Biology, Seoul National University, Seoul 151-742, Korea; the ‡Laboratory of Immunology, Korea Cancer Center Hospital, Seoul 139-240, Korea; and the §Department of Molecular Biology, Dankook University, Seoul 140-714, Korea
Myeong G. Kang
From the *Institute for Molecular Biology and Genetics and Department of Molecular Biology, Seoul National University, Seoul 151-742, Korea; the ‡Laboratory of Immunology, Korea Cancer Center Hospital, Seoul 139-240, Korea; and the §Department of Molecular Biology, Dankook University, Seoul 140-714, Korea
Young H. Kim
From the *Institute for Molecular Biology and Genetics and Department of Molecular Biology, Seoul National University, Seoul 151-742, Korea; the ‡Laboratory of Immunology, Korea Cancer Center Hospital, Seoul 139-240, Korea; and the §Department of Molecular Biology, Dankook University, Seoul 140-714, Korea
Yong H. Jin
From the *Institute for Molecular Biology and Genetics and Department of Molecular Biology, Seoul National University, Seoul 151-742, Korea; the ‡Laboratory of Immunology, Korea Cancer Center Hospital, Seoul 139-240, Korea; and the §Department of Molecular Biology, Dankook University, Seoul 140-714, Korea
Changjin Lee
From the *Institute for Molecular Biology and Genetics and Department of Molecular Biology, Seoul National University, Seoul 151-742, Korea; the ‡Laboratory of Immunology, Korea Cancer Center Hospital, Seoul 139-240, Korea; and the §Department of Molecular Biology, Dankook University, Seoul 140-714, Korea
Hee-Yong Chung
From the *Institute for Molecular Biology and Genetics and Department of Molecular Biology, Seoul National University, Seoul 151-742, Korea; the ‡Laboratory of Immunology, Korea Cancer Center Hospital, Seoul 139-240, Korea; and the §Department of Molecular Biology, Dankook University, Seoul 140-714, Korea
Hyockman Kwon
From the *Institute for Molecular Biology and Genetics and Department of Molecular Biology, Seoul National University, Seoul 151-742, Korea; the ‡Laboratory of Immunology, Korea Cancer Center Hospital, Seoul 139-240, Korea; and the §Department of Molecular Biology, Dankook University, Seoul 140-714, Korea
Sang D. Park
From the *Institute for Molecular Biology and Genetics and Department of Molecular Biology, Seoul National University, Seoul 151-742, Korea; the ‡Laboratory of Immunology, Korea Cancer Center Hospital, Seoul 139-240, Korea; and the §Department of Molecular Biology, Dankook University, Seoul 140-714, Korea
Rho H. Seong
From the *Institute for Molecular Biology and Genetics and Department of Molecular Biology, Seoul National University, Seoul 151-742, Korea; the ‡Laboratory of Immunology, Korea Cancer Center Hospital, Seoul 139-240, Korea; and the §Department of Molecular Biology, Dankook University, Seoul 140-714, Korea
Address correspondence to Dr. Rho Hyun Seong, Institute for Molecular Biology and Genetics, Seoul National University, Kwanak-gu Shinlim-dong San 56-1 Bldg. 105, Seoul 151-742, Korea.
1 Abbreviations used in this paper: GC, glucocorticoids; GR, glucocorticoid receptor; GST, glutathione-S-transferase; IP, immunoprecipitation; MACS, magnetic activated cell sorter; PI, propidium iodide.
Received:
January 22 1997
Revision Received:
March 17 1997
Online ISSN: 1540-9538
Print ISSN: 0022-1007
1997
J Exp Med (1997) 185 (10): 1827–1836.
Article history
Received:
January 22 1997
Revision Received:
March 17 1997
Citation
Sung H. Jeon, Myeong G. Kang, Young H. Kim, Yong H. Jin, Changjin Lee, Hee-Yong Chung, Hyockman Kwon, Sang D. Park, Rho H. Seong; A New Mouse Gene, SRG3, Related to the SWI3 of Saccharomyces cerevisiae, Is Required for Apoptosis Induced by Glucocorticoids in a Thymoma Cell Line. J Exp Med 19 May 1997; 185 (10): 1827–1836. doi: https://doi.org/10.1084/jem.185.10.1827
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