Herpesviral DNA fragments isolated from AIDS-associated Kaposi's sarcoma (KS) tissue (KSHV-DNA) share homology with two lymphotropic oncogenic gamma-herpesviruses, Epstein-Barr virus and Herpesvirus saimiri, and are present in the lesions of more than 95% of HIV and non-HIV-associated forms of KS, AIDS-related body cavity-based lymphomas, and AIDS-related multicentric Castleman's disease. Here we show that BC-1, a KSHV-DNA-positive, body cavity-based lymphoma cell line, produces infective herpesviral particles carrying a linear 270-kb genome that specifically transmits KSHV-DNA to CD19+ B cells. Transmission of KSHV-DNA is dependent upon a biologically active, replicating virus, since it is blocked by UV irradiation and foscarnet, an inhibitor of viral DNA-polymerase. This study represents the first isolation and transmission of the human herpesvirus-8/KS-associated herpesvirus.
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1 May 1996
Article|
May 01 1996
Human herpesvirus-8/Kaposi's sarcoma-associated herpesvirus is a new transmissible virus that infects B cells.
E A Mesri,
E A Mesri
Department of Medicine, Cornell University Medical College, New York 10021, USA.
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E Cesarman,
E Cesarman
Department of Medicine, Cornell University Medical College, New York 10021, USA.
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L Arvanitakis,
L Arvanitakis
Department of Medicine, Cornell University Medical College, New York 10021, USA.
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S Rafii,
S Rafii
Department of Medicine, Cornell University Medical College, New York 10021, USA.
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M A Moore,
M A Moore
Department of Medicine, Cornell University Medical College, New York 10021, USA.
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D N Posnett,
D N Posnett
Department of Medicine, Cornell University Medical College, New York 10021, USA.
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D M Knowles,
D M Knowles
Department of Medicine, Cornell University Medical College, New York 10021, USA.
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A S Asch
A S Asch
Department of Medicine, Cornell University Medical College, New York 10021, USA.
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E A Mesri
Department of Medicine, Cornell University Medical College, New York 10021, USA.
E Cesarman
Department of Medicine, Cornell University Medical College, New York 10021, USA.
L Arvanitakis
Department of Medicine, Cornell University Medical College, New York 10021, USA.
S Rafii
Department of Medicine, Cornell University Medical College, New York 10021, USA.
M A Moore
Department of Medicine, Cornell University Medical College, New York 10021, USA.
D N Posnett
Department of Medicine, Cornell University Medical College, New York 10021, USA.
D M Knowles
Department of Medicine, Cornell University Medical College, New York 10021, USA.
A S Asch
Department of Medicine, Cornell University Medical College, New York 10021, USA.
Online ISSN: 1540-9538
Print ISSN: 0022-1007
J Exp Med (1996) 183 (5): 2385–2390.
Citation
E A Mesri, E Cesarman, L Arvanitakis, S Rafii, M A Moore, D N Posnett, D M Knowles, A S Asch; Human herpesvirus-8/Kaposi's sarcoma-associated herpesvirus is a new transmissible virus that infects B cells.. J Exp Med 1 May 1996; 183 (5): 2385–2390. doi: https://doi.org/10.1084/jem.183.5.2385
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