Two-dimensional gel electrophoresis of in vitro phosphorylated proteins coprecipitated by CD2 monoclonal antibody (mAb) from Brij58 lysates of resting human T lymphocytes and natural killer (NK) cells resulted in the identification of a novel 29/30-kD disulfide-linked dimer (pp29/30). Comparative two-dimensional analysis of CD2, CD3, CD4, CD5, and CD8 immunoprecipitates revealed that pp29/30 associates with these signaling receptor complexes but not with CD18, CD27, and CD29 in human T lymphocytes. Analysis of CD2 immunoprecipitates prepared from T cell antigen receptor/CD3-modulated T lymphocytes indicated that pp29/30 preferentially associates and comodulates with the human T cell antigen receptor (TCR). Since tyrosine phosphorylated pp29/30 selectively interacts with the Src homology type 2 domains (SHZ) of the protein tyrosine kinases p56lck and p59fyn but not ZAP70 the present data suggest that pp29/30 represents a novel signaling receptor associated phosphoprotein likely involved in the activation of human T lymphocytes and NK cells.
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1 September 1994
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September 01 1994
Identification of a novel dimeric phosphoprotein (PP29/30) associated with signaling receptors in human T lymphocytes and natural killer cells.
B Schraven,
B Schraven
Department of Applied Immunology, Deutsches Krebsforschungszentrum, Heidelberg, Germany.
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S Ratnofsky,
S Ratnofsky
Department of Applied Immunology, Deutsches Krebsforschungszentrum, Heidelberg, Germany.
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Y Gaumont,
Y Gaumont
Department of Applied Immunology, Deutsches Krebsforschungszentrum, Heidelberg, Germany.
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H Lindegger,
H Lindegger
Department of Applied Immunology, Deutsches Krebsforschungszentrum, Heidelberg, Germany.
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H Kirchgessner,
H Kirchgessner
Department of Applied Immunology, Deutsches Krebsforschungszentrum, Heidelberg, Germany.
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E Bruyns,
E Bruyns
Department of Applied Immunology, Deutsches Krebsforschungszentrum, Heidelberg, Germany.
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U Moebius,
U Moebius
Department of Applied Immunology, Deutsches Krebsforschungszentrum, Heidelberg, Germany.
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S C Meuer
S C Meuer
Department of Applied Immunology, Deutsches Krebsforschungszentrum, Heidelberg, Germany.
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B Schraven
Department of Applied Immunology, Deutsches Krebsforschungszentrum, Heidelberg, Germany.
S Ratnofsky
Department of Applied Immunology, Deutsches Krebsforschungszentrum, Heidelberg, Germany.
Y Gaumont
Department of Applied Immunology, Deutsches Krebsforschungszentrum, Heidelberg, Germany.
H Lindegger
Department of Applied Immunology, Deutsches Krebsforschungszentrum, Heidelberg, Germany.
H Kirchgessner
Department of Applied Immunology, Deutsches Krebsforschungszentrum, Heidelberg, Germany.
E Bruyns
Department of Applied Immunology, Deutsches Krebsforschungszentrum, Heidelberg, Germany.
U Moebius
Department of Applied Immunology, Deutsches Krebsforschungszentrum, Heidelberg, Germany.
S C Meuer
Department of Applied Immunology, Deutsches Krebsforschungszentrum, Heidelberg, Germany.
Online ISSN: 1540-9538
Print ISSN: 0022-1007
J Exp Med (1994) 180 (3): 897–906.
Citation
B Schraven, S Ratnofsky, Y Gaumont, H Lindegger, H Kirchgessner, E Bruyns, U Moebius, S C Meuer; Identification of a novel dimeric phosphoprotein (PP29/30) associated with signaling receptors in human T lymphocytes and natural killer cells.. J Exp Med 1 September 1994; 180 (3): 897–906. doi: https://doi.org/10.1084/jem.180.3.897
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