We investigated the role of the complementarity determining region 1 (CDR1) of T cell receptor (TCR) beta chain both in antigen/major histocompatibility complex I (MHC I) and in superantigen (SAg)/MHC II complex recognition. Residues 26 to 31 of the V beta 10 domain of a TCR derived from an H-2Kd-restricted cytotoxic clone were individually changed to alanine, using site-directed mutagenesis, and the mutated TCR beta chains were transfected along with the wild-type TCR alpha chain into a TCR alpha-beta-T hydridoma. These mutations affected antigen/H-2Kd complex recognition, although to a different extent, as estimated by interleukin 2 production. Certain mutations also affected differently the recognition of two Staphylococcal toxins, exfoliative toxin and Staphylococcal enterotoxin C2, presented by HLA-DR1. Whereas mutation of residues D30 or T31 affect the recognition of both toxins, residues T26, L27, and H29 are critical for the recognition of only one of the SAgs. These observations demonstrate the participation of the CDR1 region in the recognition of peptide/MHC class I as well as SAg/MHC II complexes.
Article|
April 01 1994
The V beta complementarity determining region 1 of a major histocompatibility complex (MHC) class I-restricted T cell receptor is involved in the recognition of peptide/MHC I and superantigen/MHC II complex.
M Bellio,
M Bellio
Unité Institut National de la Santé et de la Recherche Médicale (INSERM) 277, Department of Immunology, Institut Pasteur, Paris, France.
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Y C Lone,
Y C Lone
Unité Institut National de la Santé et de la Recherche Médicale (INSERM) 277, Department of Immunology, Institut Pasteur, Paris, France.
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O de la Calle-Martin,
O de la Calle-Martin
Unité Institut National de la Santé et de la Recherche Médicale (INSERM) 277, Department of Immunology, Institut Pasteur, Paris, France.
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B Malissen,
B Malissen
Unité Institut National de la Santé et de la Recherche Médicale (INSERM) 277, Department of Immunology, Institut Pasteur, Paris, France.
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J P Abastado,
J P Abastado
Unité Institut National de la Santé et de la Recherche Médicale (INSERM) 277, Department of Immunology, Institut Pasteur, Paris, France.
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P Kourilsky
P Kourilsky
Unité Institut National de la Santé et de la Recherche Médicale (INSERM) 277, Department of Immunology, Institut Pasteur, Paris, France.
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M Bellio
Unité Institut National de la Santé et de la Recherche Médicale (INSERM) 277, Department of Immunology, Institut Pasteur, Paris, France.
Y C Lone
Unité Institut National de la Santé et de la Recherche Médicale (INSERM) 277, Department of Immunology, Institut Pasteur, Paris, France.
O de la Calle-Martin
Unité Institut National de la Santé et de la Recherche Médicale (INSERM) 277, Department of Immunology, Institut Pasteur, Paris, France.
B Malissen
Unité Institut National de la Santé et de la Recherche Médicale (INSERM) 277, Department of Immunology, Institut Pasteur, Paris, France.
J P Abastado
Unité Institut National de la Santé et de la Recherche Médicale (INSERM) 277, Department of Immunology, Institut Pasteur, Paris, France.
P Kourilsky
Unité Institut National de la Santé et de la Recherche Médicale (INSERM) 277, Department of Immunology, Institut Pasteur, Paris, France.
Online ISSN: 1540-9538
Print ISSN: 0022-1007
J Exp Med (1994) 179 (4): 1087–1097.
Citation
M Bellio, Y C Lone, O de la Calle-Martin, B Malissen, J P Abastado, P Kourilsky; The V beta complementarity determining region 1 of a major histocompatibility complex (MHC) class I-restricted T cell receptor is involved in the recognition of peptide/MHC I and superantigen/MHC II complex.. J Exp Med 1 April 1994; 179 (4): 1087–1097. doi: https://doi.org/10.1084/jem.179.4.1087
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