Whereas there is considerable information on the phenotypic and functional maturation of T cell receptor (TCR) alpha/beta thymocytes, comparatively little is known of the maturational processes that affect development of TCR-gamma/delta thymocytes. One class of gamma/delta T cells, those bearing the V gamma 3 gene product, are generated only during the early fetal stages of thymic development, and then migrate to the skin. Here we examine the intrathymic differentiation of these V gamma 3+ cells. The earliest V gamma 3 cells to appear in the thymus expressed low levels of TCR (V gamma 3low) and high levels of heat stable antigen (HSA). Over the next few days, V gamma 3+ thymocytes appeared which expressed high levels of TCR (V gamma 3high) and very low levels of HSA. The antigens CD5, CD45RB, and MEL14 were also differentially expressed on V gamma 3low versus V gamma 3high thymocytes, but the shift in expression was the opposite as compared with immature and mature TCR-alpha/beta thymocytes. Transfer experiments of sorted V gamma 3low/HSAhigh thymocytes to SCID thymic lobes showed that these cells were indeed the precursors of V gamma 3high/HSAlow thymocytes. The phenotype of the V gamma 3high thymocytes was similar to that of the postthymic V gamma 3+ cells found in the skin of adult mice. The differentiation of V gamma 3low in V gamma 3high thymocytes was also observed in fetal thymic organ culture. Addition of cyclosporin A (CsA) to these cultures had little effect on the appearance of V gamma 3low/HSAhigh cells, but blocked the appearance of V gamma 3high/HSAlow cells. These results show that, like alpha/beta T cells, V gamma 3+ thymocytes differentiate from TCRlow precursors to cells with a mature phenotype and that CsA inhibits this transition.
Skip Nav Destination
Article navigation
1 July 1993
Article|
July 01 1993
Intrathymic differentiation of V gamma 3 T cells.
G Leclercq,
G Leclercq
Laboratory of Bacteriology, Virology and Immunology, University Hospital Gent, Belgium.
Search for other works by this author on:
J Plum,
J Plum
Laboratory of Bacteriology, Virology and Immunology, University Hospital Gent, Belgium.
Search for other works by this author on:
D Nandi,
D Nandi
Laboratory of Bacteriology, Virology and Immunology, University Hospital Gent, Belgium.
Search for other works by this author on:
M De Smedt,
M De Smedt
Laboratory of Bacteriology, Virology and Immunology, University Hospital Gent, Belgium.
Search for other works by this author on:
J P Allison
J P Allison
Laboratory of Bacteriology, Virology and Immunology, University Hospital Gent, Belgium.
Search for other works by this author on:
G Leclercq
Laboratory of Bacteriology, Virology and Immunology, University Hospital Gent, Belgium.
J Plum
Laboratory of Bacteriology, Virology and Immunology, University Hospital Gent, Belgium.
D Nandi
Laboratory of Bacteriology, Virology and Immunology, University Hospital Gent, Belgium.
M De Smedt
Laboratory of Bacteriology, Virology and Immunology, University Hospital Gent, Belgium.
J P Allison
Laboratory of Bacteriology, Virology and Immunology, University Hospital Gent, Belgium.
Online ISSN: 1540-9538
Print ISSN: 0022-1007
J Exp Med (1993) 178 (1): 309–315.
Citation
G Leclercq, J Plum, D Nandi, M De Smedt, J P Allison; Intrathymic differentiation of V gamma 3 T cells.. J Exp Med 1 July 1993; 178 (1): 309–315. doi: https://doi.org/10.1084/jem.178.1.309
Download citation file:
Sign in
Don't already have an account? Register
Client Account
You could not be signed in. Please check your email address / username and password and try again.
Could not validate captcha. Please try again.
Sign in via your Institution
Sign in via your InstitutionSuggested Content
Email alerts
Advertisement