A synthetic peptide based on a sequence containing thyroxine at position 2553 in thyroglobulin (Tg), and already shown to be recognized by two clonotypically distinct murine Tg autoreactive T cell hybridomas, can trigger primed lymph node cells to transfer thyroiditis to naive recipients. Donor lymph node cells could be prepared from mice immunized either with intact mouse Tg or with this peptide itself. After a second exposure to the priming antigen in vitro, both these populations induced 100% thyroiditis in recipient animals. The importance of the T4 residue in the development of disease was demonstrated by the failure of Tg tryptic peptides depleted of T4 to stimulate pathogenic effectors in vitro, even when the lymph node cells had been taken from mice primed with whole Tg. We conclude that this T4-containing 12mer sequence is a major thyroiditogenic epitope in CBA/J mice although we cannot exclude the possibility that there are other pathogenic epitopes present in the whole Tg molecule.
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1 March 1992
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March 01 1992
A thyroxine-containing peptide can induce murine experimental autoimmune thyroiditis.
P R Hutchings,
P R Hutchings
Immunology Department, University College and Middlesex Medical School, London, United Kingdom.
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A Cooke,
A Cooke
Immunology Department, University College and Middlesex Medical School, London, United Kingdom.
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K Dawe,
K Dawe
Immunology Department, University College and Middlesex Medical School, London, United Kingdom.
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B R Champion,
B R Champion
Immunology Department, University College and Middlesex Medical School, London, United Kingdom.
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M Geysen,
M Geysen
Immunology Department, University College and Middlesex Medical School, London, United Kingdom.
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R Valerio,
R Valerio
Immunology Department, University College and Middlesex Medical School, London, United Kingdom.
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I M Roitt
I M Roitt
Immunology Department, University College and Middlesex Medical School, London, United Kingdom.
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P R Hutchings
Immunology Department, University College and Middlesex Medical School, London, United Kingdom.
A Cooke
Immunology Department, University College and Middlesex Medical School, London, United Kingdom.
K Dawe
Immunology Department, University College and Middlesex Medical School, London, United Kingdom.
B R Champion
Immunology Department, University College and Middlesex Medical School, London, United Kingdom.
M Geysen
Immunology Department, University College and Middlesex Medical School, London, United Kingdom.
R Valerio
Immunology Department, University College and Middlesex Medical School, London, United Kingdom.
I M Roitt
Immunology Department, University College and Middlesex Medical School, London, United Kingdom.
Online ISSN: 1540-9538
Print ISSN: 0022-1007
J Exp Med (1992) 175 (3): 869–872.
Citation
P R Hutchings, A Cooke, K Dawe, B R Champion, M Geysen, R Valerio, I M Roitt; A thyroxine-containing peptide can induce murine experimental autoimmune thyroiditis.. J Exp Med 1 March 1992; 175 (3): 869–872. doi: https://doi.org/10.1084/jem.175.3.869
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