In an attempt to identify a molecule in target recognition by CD3- large granular lymphocytes (LGL), we have generated a rabbit antiidiotypic (anti-ID) serum against a monoclonal antibody (mAb 36) that reacted with the cell membrane of K562. Flow cytometry analysis demonstrated that the anti-ID serum bound selectively to CD3- LGL and that F(ab')2 fragments of the anti-ID serum blocked both target cell binding and lysis by NK cells. Stimulation of CD3- LGL with F(ab')2 fragments resulted in the release of serine esterases and the secretion of interferon gamma. Furthermore, anti-ID F(ab')2 antibodies crosslinked to anti-DNP F(ab')2 mediated directed cytotoxicity of a non-natural killer (NK)-susceptible mouse target (YAC-1) via this surface ligand. These functional reactivities were only removed by adsorption with the specific idiotype. Protein analysis showed that the anti-ID serum immunoprecipitated 80-, 110-, and 150-kD proteins. Using this anti-ID, a partial cDNA was cloned and an antipeptide antiserum was made against the portion of the predicted amino acid sequence that corresponded to a portion of the ID binding region. This antipeptide serum exhibited similar functional and biochemical reactivities to those observed with the anti-ID serum. These data suggest that the cell surface moiety recognized by the anti-ID and anti-p104 is novel and is selectively involved in both recognition and triggering of NK-mediated lytic function.
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1 December 1991
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December 01 1991
Mechanism of target cell recognition by natural killer cells: characterization of a novel triggering molecule restricted to CD3- large granular lymphocytes.
J L Frey,
J L Frey
Laboratory of Experimental Immunology, National Cancer Institute, Frederick Cancer Research and Development Center, Maryland 21702.
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T Bino,
T Bino
Laboratory of Experimental Immunology, National Cancer Institute, Frederick Cancer Research and Development Center, Maryland 21702.
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R R Kantor,
R R Kantor
Laboratory of Experimental Immunology, National Cancer Institute, Frederick Cancer Research and Development Center, Maryland 21702.
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D M Segal,
D M Segal
Laboratory of Experimental Immunology, National Cancer Institute, Frederick Cancer Research and Development Center, Maryland 21702.
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S L Giardina,
S L Giardina
Laboratory of Experimental Immunology, National Cancer Institute, Frederick Cancer Research and Development Center, Maryland 21702.
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J Roder,
J Roder
Laboratory of Experimental Immunology, National Cancer Institute, Frederick Cancer Research and Development Center, Maryland 21702.
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S Anderson,
S Anderson
Laboratory of Experimental Immunology, National Cancer Institute, Frederick Cancer Research and Development Center, Maryland 21702.
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J R Ortaldo
J R Ortaldo
Laboratory of Experimental Immunology, National Cancer Institute, Frederick Cancer Research and Development Center, Maryland 21702.
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J L Frey
Laboratory of Experimental Immunology, National Cancer Institute, Frederick Cancer Research and Development Center, Maryland 21702.
T Bino
Laboratory of Experimental Immunology, National Cancer Institute, Frederick Cancer Research and Development Center, Maryland 21702.
R R Kantor
Laboratory of Experimental Immunology, National Cancer Institute, Frederick Cancer Research and Development Center, Maryland 21702.
D M Segal
Laboratory of Experimental Immunology, National Cancer Institute, Frederick Cancer Research and Development Center, Maryland 21702.
S L Giardina
Laboratory of Experimental Immunology, National Cancer Institute, Frederick Cancer Research and Development Center, Maryland 21702.
J Roder
Laboratory of Experimental Immunology, National Cancer Institute, Frederick Cancer Research and Development Center, Maryland 21702.
S Anderson
Laboratory of Experimental Immunology, National Cancer Institute, Frederick Cancer Research and Development Center, Maryland 21702.
J R Ortaldo
Laboratory of Experimental Immunology, National Cancer Institute, Frederick Cancer Research and Development Center, Maryland 21702.
Online ISSN: 1540-9538
Print ISSN: 0022-1007
J Exp Med (1991) 174 (6): 1527–1536.
Citation
J L Frey, T Bino, R R Kantor, D M Segal, S L Giardina, J Roder, S Anderson, J R Ortaldo; Mechanism of target cell recognition by natural killer cells: characterization of a novel triggering molecule restricted to CD3- large granular lymphocytes.. J Exp Med 1 December 1991; 174 (6): 1527–1536. doi: https://doi.org/10.1084/jem.174.6.1527
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