Prior studies identified a segment of the CD2 cytoplasmic domain between amino acid (aa) residues 253 and 287 as important in T lymphocyte signal transduction. This region contains two repeats of the sequence motif PPPGHR, thought to form a "cage" structure involved in CD2-mediated signaling. To evaluate this segment, a series of mutant human CD2 molecules were produced by oligonucleotide-directed mutagenesis and inserted into the ovalbumin-specific, I-Ad-restricted murine T-T hybridoma 3DO54.8 using the DOL retroviral system. CD2 M1 (271-272), CD2 M2 (278-279), and CD2 M4 (264-265) mutants replaced the positively charged adjacent aa histidine and arginine (HR) in the wild-type CD2 sequence with aspartic and glutamic acid (DE) at positions 271-272, 278-279, and 264-265, respectively. In addition, a truncation mutant, CD2 M3 (268), containing only 57 of the 117 cytoplasmic aa and terminating before the second PPPGHR sequence, was generated. Stimulation of transfectants CD2 FL, CD2 M1 (271-272), and CD2 M2 (278-279) with anti-T11(2) + anti-T11(3) antibodies resulted in a rise in cytosolic-free calcium [( Ca2+]i) and subsequent interleukin 2 (IL-2) secretion. In contrast, CD2 M4 (264-265) transfectants could not be activated in either assay. Thus, alteration of histidine 264 and/or arginine 265 within the first PPPGHR motif affects the process of signal transduction via CD2, whereas identical mutations in residues at 271-272 or 278-279 were individually without effect. Consistent with these data, CD2 M3 (268) transfectants were able to generate a detectable amount of IL-2 via CD2 triggering. These data support the notion that the PPPGHR motif at aa 260-265 is important for activation of T lymphocytes via the CD2 molecule.
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1 July 1990
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July 01 1990
Involvement of the PPPGHR motif in T cell activation via CD2.
H C Chang,
H C Chang
Laboratory of Immunobiology, Dana-Farber Cancer Institute, Boston, Massachusetts.
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P Moingeon,
P Moingeon
Laboratory of Immunobiology, Dana-Farber Cancer Institute, Boston, Massachusetts.
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R Pedersen,
R Pedersen
Laboratory of Immunobiology, Dana-Farber Cancer Institute, Boston, Massachusetts.
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J Lucich,
J Lucich
Laboratory of Immunobiology, Dana-Farber Cancer Institute, Boston, Massachusetts.
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C Stebbins,
C Stebbins
Laboratory of Immunobiology, Dana-Farber Cancer Institute, Boston, Massachusetts.
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E L Reinherz
E L Reinherz
Laboratory of Immunobiology, Dana-Farber Cancer Institute, Boston, Massachusetts.
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H C Chang
Laboratory of Immunobiology, Dana-Farber Cancer Institute, Boston, Massachusetts.
P Moingeon
Laboratory of Immunobiology, Dana-Farber Cancer Institute, Boston, Massachusetts.
R Pedersen
Laboratory of Immunobiology, Dana-Farber Cancer Institute, Boston, Massachusetts.
J Lucich
Laboratory of Immunobiology, Dana-Farber Cancer Institute, Boston, Massachusetts.
C Stebbins
Laboratory of Immunobiology, Dana-Farber Cancer Institute, Boston, Massachusetts.
E L Reinherz
Laboratory of Immunobiology, Dana-Farber Cancer Institute, Boston, Massachusetts.
Online ISSN: 1540-9538
Print ISSN: 0022-1007
J Exp Med (1990) 172 (1): 351–355.
Citation
H C Chang, P Moingeon, R Pedersen, J Lucich, C Stebbins, E L Reinherz; Involvement of the PPPGHR motif in T cell activation via CD2.. J Exp Med 1 July 1990; 172 (1): 351–355. doi: https://doi.org/10.1084/jem.172.1.351
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