We have analyzed in detail the precise requirements for the induction of human IgE synthesis using several experimental approaches with purified B cells and well-characterized alloantigen-specific CD4+ T cell clones expressing different profiles of lymphokine secretion. Using these clones under cognate conditions in which the B cells expressed alloantigens recognized by the cloned T cells, we have confirmed that IL-4 is required for the induction of IgE synthesis, but we have clearly demonstrated that IL-4 by itself is not sufficient. With several cloned CD4+ T cell lines, including an IL-4-producing clone that could not induce IgE synthesis, and cloned T cells pretreated with cyclosporin A to inhibit lymphokine synthesis, we showed that Th cell-B cell interactions are necessary for IgE synthesis, and that low molecular weight B cell growth factor (LMW-BCGF) and IL-4, in combination, are lymphokines of major importance in the induction of IgE synthesis. Together our results indicate that optimal induction of an IgE-specific response requires the exposure of B cells to a particular complex of signals that include (a) a signal(s) involving Th-B cell interaction that primes B cells to receive additional signals from soluble lymphokines, (b) a specific B cell proliferative signal provided by LMW-BCGF, and (c) a specific B cell differentiation signal provided by IL-4.
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1 November 1989
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November 01 1989
Induction of human IgE synthesis by CD4+ T cell clones. Requirement for interleukin 4 and low molecular weight B cell growth factor.
R H DeKruyff,
R H DeKruyff
Department of Pediatrics, Stanford University School of Medicine, California 94305.
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T Turner,
T Turner
Department of Pediatrics, Stanford University School of Medicine, California 94305.
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J S Abrams,
J S Abrams
Department of Pediatrics, Stanford University School of Medicine, California 94305.
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M A Palladino, Jr,
M A Palladino, Jr
Department of Pediatrics, Stanford University School of Medicine, California 94305.
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D T Umetsu
D T Umetsu
Department of Pediatrics, Stanford University School of Medicine, California 94305.
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R H DeKruyff
Department of Pediatrics, Stanford University School of Medicine, California 94305.
T Turner
Department of Pediatrics, Stanford University School of Medicine, California 94305.
J S Abrams
Department of Pediatrics, Stanford University School of Medicine, California 94305.
M A Palladino, Jr
Department of Pediatrics, Stanford University School of Medicine, California 94305.
D T Umetsu
Department of Pediatrics, Stanford University School of Medicine, California 94305.
Online ISSN: 1540-9538
Print ISSN: 0022-1007
J Exp Med (1989) 170 (5): 1477–1493.
Citation
R H DeKruyff, T Turner, J S Abrams, M A Palladino, D T Umetsu; Induction of human IgE synthesis by CD4+ T cell clones. Requirement for interleukin 4 and low molecular weight B cell growth factor.. J Exp Med 1 November 1989; 170 (5): 1477–1493. doi: https://doi.org/10.1084/jem.170.5.1477
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